Document Detail

Performance of free beta-human chorionic gonadotrophin (free beta-hCG) and pregnancy associated plasma protein-A (PAPP-A) analysis between Delfia Xpress and AutoDelfia systems in The Netherlands.
MedLine Citation:
PMID:  19191730     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The VU University Medical Center (VUmc) was the first hospital in the Netherlands to introduce the Delfia Xpress for the analysis of free beta-human chorionic gonadotrophin (beta-hCG) and pregnancy associated plasma protein-A (PAPP-A) in the first trimester screening program for Down syndrome. Since then, others have implemented this system. In this study, we tested the equality of measurements for free beta-hCG and PAPP-A between Delfia Xpress systems and one AutoDelfia system. METHODS: A total of 40 serum samples were aliquoted and stored at -20 degrees C. Samples were analyzed by six Delfia Xpress systems and one AutoDelfia system over a time period of 2 years. RESULTS: The relationships between free beta-hCG and PAPP-A were excellent for the different Delfia Xpress systems (r>0.99, p<0.0001). For PAPP-A, the agreement between the main system at VUmc and five other systems was linear with slopes between 0.99 and 1.06. Similarly, agreement for free beta-hCG was linear with slopes between 0.99 and 1.09. Likewise, agreement for PAPP-A and free beta-hCG was excellent for the AutoDelfia vs. the main Delfia Xpress at the VUmc (r>0.99, p<0.0001). For both PAPP-A and free beta-hCG, the relationships were linear with slopes of 1.08 and 1.07. CONCLUSIONS: We demonstrate an excellent agreement for the analysis of PAPP-A and free beta-hCG between Delfia Xpress systems and one AutoDelfia system.
Ingeborg H Linskens; Marieke Levitus; Anneke Frans; Peter C J I Schielen; John M G van Vugt; Marinus A Blankenstein; Hilde M Dijstelbloem
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry and laboratory medicine : CCLM / FESCC     Volume:  47     ISSN:  1434-6621     ISO Abbreviation:  Clin. Chem. Lab. Med.     Publication Date:  2009  
Date Detail:
Created Date:  2009-02-04     Completed Date:  2009-05-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806306     Medline TA:  Clin Chem Lab Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  222-6     Citation Subset:  IM    
Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands.
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MeSH Terms
Chorionic Gonadotropin, beta Subunit, Human / blood*
Down Syndrome / blood,  diagnosis
Immunoassay / instrumentation,  methods*
Predictive Value of Tests
Pregnancy-Associated Plasma Protein-A / analysis*
Reagent Kits, Diagnostic
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Chorionic Gonadotropin, beta Subunit, Human; 0/Reagent Kits, Diagnostic; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

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