| Performance evaluation of phycocyanin probes for the monitoring of cyanobacteria. | |
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MedLine Citation:
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PMID: 21103573 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The performance of two field probes (YSI 6600 and TriOS), used for the measurement of in vivo phycocyanin fluorescence, was compared and validated in the laboratory in 2008 and 2009 with cultures of Microcystis aeruginosa and field samples. The background noise of the two probes was low and the detection limits were estimated at 1500 cells mL(-1) for the YSI and 0.69 µg PC L(-1) for the TriOS. The linearity and repeatability of both probes have been excellent. Strong relationships were observed between the in vivo fluorescence and the total cyanobacterial biovolume (R(2) = 0.82 YSI; 0.83 TriOS) or the abundance (R(2) = 0.71 YSI; 0.75 TriOS) of cyanobacteria. However, the difference between cell densities determined by microscopy and measured by the YSI can be very large and has been associated to the variability of cell volume among cyanobacteria. This last observation makes the YSI a qualitative tool if a post-calibration is not done. The analysis of filtrated samples showed that dissolved phycocyanin (extracellular) may represent a significant fluorescence signal. No relationship could be established between the abundance, the total cyanobacterial biovolume or the in vivo fluorescence of phycocyanin and the concentrations of cyanotoxins (R(2) ≤ 0.22). |
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Authors:
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Christian Bastien; Richard Cardin; Eloïse Veilleux; Christian Deblois; Annabelle Warren; Isabelle Laurion |
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Publication Detail:
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Type: Journal Article Date: 2010-10-26 |
Journal Detail:
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Title: Journal of environmental monitoring : JEM Volume: 13 ISSN: 1464-0333 ISO Abbreviation: J Environ Monit Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100968688 Medline TA: J Environ Monit Country: England |
Other Details:
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Languages: eng Pagination: 110-8 Citation Subset: IM |
Affiliation:
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Centre d'expertise en analyse environnementale, MDDEP, 2700 rue Einstein, Québec (Qc), G1P 3W8, Canada. christian.bastien@mddep.gouv.qc.ca |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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