Document Detail

Performance of a component-based allergen-microarray in the diagnosis of cow's milk and hen's egg allergy.
MedLine Citation:
PMID:  20633029     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The food challenge test (FCT) is the gold standard for the diagnosis of food allergy. This procedure is time consuming, costly and can induce potentially severe symptoms. An ideal in vitro test should allow to avoid the FCT. Objective To assess the clinical performance of microarray for specific IgE (sIgE) detection in children with challenge-proven/excluded cow's milk (CM) or hen's egg (HE) allergy.
METHODS: One-hundred and four children with suspected IgE-mediated hypersensitivity to CM or HE were studied. In all patients, skin prick test, ImmunoCAP, microarray and FCT were performed.
RESULTS: The microarray components Bos d 8 for CM (27/58 patients) and Gal d 1 (20/46 patients) and Gal d 2 (24/46) for HE were the most frequently recognized allergens. Using the FCT results as the reference parameter, sIgE to Bos d 8 and Gal d 1 had the highest area under the curves. These were not significantly different from those obtained using the ImmunoCAP. Use of 95% clinical decision points (CDP) for sIgE to Bos d 8 and Gal d 1 resulted in higher negative predictive values (78% and 79%, respectively) than those obtained with the ImmunoCAP (57% and 59%).
CONCLUSIONS: Our results show that in children with suspected CM or HE allergy, the microarray has a good ability to predict the FCT results. In a clinical application perspective, the microarray could be used as a second-level assay, if the ImmunoCAP sIgE is <95% CDP. This approach would lead to a decrease in the number of the FCT to be performed, as well as of positive FCTs with a subsequent decrease in severe reaction risk.
L E D'Urbano; K Pellegrino; M C Artesani; S Donnanno; R Luciano; C Riccardi; A E Tozzi; L Ravà; F De Benedetti; G Cavagni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  40     ISSN:  1365-2222     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-12     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  1561-70     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Rheumatology Research Laboratory, IRCCS Children's Hospital Bambino Gesù, Rome, Italy.
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MeSH Terms
Allergens / analysis*,  immunology
Area Under Curve
Child, Preschool
Egg Hypersensitivity / diagnosis*,  immunology
Enzyme-Linked Immunosorbent Assay
Immunoglobulin E / blood,  immunology
Milk Hypersensitivity / diagnosis*,  immunology
Protein Array Analysis / methods*
ROC Curve
Sensitivity and Specificity
Skin Tests
Reg. No./Substance:
0/Allergens; 37341-29-0/Immunoglobulin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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