| Performance characteristics of a microfluidic Lab-on-Chip electrophoresis system for high-density lipoprotein (HDL) subfraction separation and measurement. | |
| | |
MedLine Citation:
|
PMID: 20298140 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: High-density lipoprotein (HDL) is believed to be protective against coronary heart disease (CHD). HDL is comprised of different subfractions. Of these, HDL 2b is believed to be the most important in preventing CHD. Current methods for HDL subfraction measurements are not standardized and often exhibit poor analytical performance, which can limit their usefulness in clinical practice. METHODS: We have developed a microfluidic Lab-on-Chip electrophoresis system for serum HDL subfraction measurements. Linear polymers are used as a sieving buffer for HDL particle separation. Nine samples and two controls can be analyzed in 30 min. The percent HDL 2b that comprises total high-density lipoprotein cholesterol (HDL-C) is reported. RESULTS: Samples with HDL-C of 0.259-2.072 mmol/L could be evaluated for percent HDL 2b with coefficient of variations (%CVs) below 8%. Total precision was typically below 1.5% and linearity was observed between 8% and 32% HDL 2b. Measurements were not affected by many therapeutic and biological compounds. Consistent results were obtained by three laboratories and showed r(2) values between 0.93 and 0.95. CONCLUSIONS: The assay allows for easy and reproducible measurement of percent HDL 2b. The experimental procedure and small size of the instrumentation needed for measurement make it viable in clinical settings. |
| | |
Authors:
|
Dan-Hui D Yang; Yves Konigshofer; Jude Dike; Elaine Chang; Odilo Mueller |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 48 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-03 Completed Date: 2010-09-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
|
Languages: eng Pagination: 883-9 Citation Subset: IM |
Affiliation:
|
Agilent Technologies, Inc., Santa Clara, CA 95051, USA. dorothy_yang@agilent.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cholesterol, HDL
/
blood Coronary Disease / blood, diagnosis Electrophoresis Humans Lipoproteins, HDL / blood*, isolation & purification Microfluidic Analytical Techniques / methods* |
| Chemical | |
Reg. No./Substance:
|
0/Cholesterol, HDL; 0/Lipoproteins, HDL |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Review: Rhabdomyolysis: historical background, clinical, diagnostic and therapeutic features.
Next Document: Unilateral hand-foot syndrome: an extraordinary side effect of capecitabine.