| Perfluorinated alginate for cellular encapsulation. | |
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MedLine Citation:
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PMID: 22544596 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg-PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg-PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg-PFC hydrogel beads fabricated via Ba(2+) crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg-PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg-PFC hydrogels retain some of the PFC's biological-relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three-dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012. |
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Authors:
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Kerim M Gattás-Asfura; Christopher A Fraker; Cherie L Stabler |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-28 |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: - ISSN: 1552-4965 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Diabetes Research Institute, Miller School of Medicine, Leonard M. Miller School of Medicine, University of Miami, 1450 NW 10th Avenue, Miami, Florida 33136. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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