Document Detail


Perflubron emulsion increases subcutaneous tissue oxygen tension in rats.
MedLine Citation:
PMID:  16476072     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxygen plays a central role in wound healing. The hypothesis of this study was that the administration of a perfluorocarbon emulsion, which dissolves oxygen will increase subcutaneous tissue oxygen tension (PsqO2) in normovolemic and hemorrhaged animals. In the first set of experiments, PsqO2 was measured with a polarographic oxygen electrode along the dorsum of normovolemic-anesthetized rats (n=20) breathing supplemental oxygen. After baseline equilibration of tissue oxygen tension, perflubron emulsion (n=12) or saline control (n=8) was administered. Perflubron administration increased PsqO2 by 32.0 +/- 7.2 mmHg, whereas saline administration had no effect (-1.0 +/- 6.6 mmHg). In a second set of experiments, PsqO2 (n=12) was measured in rats breathing 100 percent oxygen after being bled 20 percent of their blood volume. Creating hypovolemic rats allowed for the study of the effect of perflubron emulsion administration on subcutaneous oxygen tension in tissues with compromised blood flow. Perflubron emulsion was only effective at increasing oxygen tension in tissues with oxygen extractions less then 2.0 vol%. These findings agree with those results analytically predicted based on the oxygen solubility of the perflubron emulsion.
Authors:
Noah A Rosen; Harriet W Hopf; Thomas K Hunt
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  14     ISSN:  1067-1927     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2006 Jan-Feb
Date Detail:
Created Date:  2006-02-14     Completed Date:  2006-08-31     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-60     Citation Subset:  IM    
Affiliation:
Department of Surgery, Boston University Medical Center, Boston, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Emulsions
Fluorocarbons / pharmacology*
Hemodynamics / drug effects
Hypovolemia / metabolism
Injections
Oxygen / metabolism*
Oxygen Consumption
Rats
Rats, Sprague-Dawley
Subcutaneous Tissue / metabolism*
Grant Support
ID/Acronym/Agency:
P50 GM27345/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Emulsions; 0/Fluorocarbons; 423-55-2/perflubron; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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