Document Detail

Percutaneous collection of fetal fluids for detection of bovine viral diarrhea virus infection in cattle.
MedLine Citation:
PMID:  11991414     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To develop a method for percutaneous collection of fetal fluid from cattle in the late stages of gestation and determine whether bovine viral diarrhea virus (BVDV) can be isolated from such fluids. DESIGN: Case series. ANIMALS: 169 pregnant beef cattle. PROCEDURE: Animals were restrained in a squeeze chute, and hair was clipped from a region of the right flank. Pregnancy was confirmed, and fetal fluids were identified by means of abdominal ultrasonography. Fetal fluid was collected with a spinal needle. Virus isolation was performed on fetal fluids, WBC lysates from 160 live calves, and tissues from 12 calves that died or were aborted. Blood samples collected from adult cattle were assayed with an immunoperoxidase monolayer assay. RESULTS: Fourteen animals aborted or delivered premature calves within 3 weeks after fetal fluid collection; however, it could not be determined whether this was a complication of the procedure or attributable to other factors. Results of BVDV isolation from fetal fluid samples were negative for 168 animals. However, a noncytopathic BVDV was isolated from fetal fluid obtained from a 2-year-old heifer; results of the immunoperoxidase assay of serum from this heifer were also positive, and a noncytopathic BVDV was isolated from tissue specimens from a stillborn calf produced by this heifer. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that fetal fluids can be collected percutaneously from cattle in the late stages of gestation and that virus isolation performed on fetal fluids can be used to identify fetuses infected with BVDV in utero. However, safety of the procedure could not be evaluated.
Robert J Callan; John A Schnackel; Hana Van Campen; Robert G Mortimer; Jacqueline A Cavender; Elizabeth S Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Veterinary Medical Association     Volume:  220     ISSN:  0003-1488     ISO Abbreviation:  J. Am. Vet. Med. Assoc.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-06     Completed Date:  2002-07-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7503067     Medline TA:  J Am Vet Med Assoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1348-52     Citation Subset:  IM    
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523-1620, USA.
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MeSH Terms
Abdomen / ultrasonography
Abortion, Veterinary / etiology
Amniocentesis / veterinary
Amniotic Fluid / virology*
Bovine Virus Diarrhea-Mucosal Disease / diagnosis*
Diarrhea Viruses, Bovine Viral / isolation & purification*
Fetal Diseases / diagnosis,  veterinary*,  virology
Fetus / virology
Infectious Disease Transmission, Vertical / veterinary
Prenatal Diagnosis / methods,  veterinary*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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