Document Detail


Perchloric acid treatment of human blood for quantitative endotoxin assay using synthetic chromogenic substrate for horseshoe crab clotting enzyme.
MedLine Citation:
PMID:  6289488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new reliable human blood treatment was established for quantitative endotoxin assay using synthetic chromogenic substrate [Boc-Leu-Gly-Arg-rho-nitroanilide]. Addition of perchloric acid in a final concentration of 1.25% to platelet-rich plasma or serum in a 2:1 volume ratio completely eliminated nonspecific amidase activities as well as inhibitors. By this method, the recovery of added endotoxin was nearly 100%, and was almost independent of sample dilutions and anticoagulants.
Authors:
H Tamura; T Obayashi; K Takagi; S Tanaka; C Nakahara; T Kawai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  27     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  51-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / metabolism
Animals
Chromogenic Compounds / pharmacology
Dose-Response Relationship, Drug
Endotoxins / blood*
Humans
Limulus Test*
Oligopeptides / pharmacology
Perchloric Acid / pharmacology*
Chemical
Reg. No./Substance:
0/Chromogenic Compounds; 0/Endotoxins; 0/Oligopeptides; 68223-96-1/butyloxycarbonyl-leucyl-glycyl-arginine-4-nitroanilide; 7601-90-3/Perchloric Acid; EC 3.5.-/Amidohydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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