Document Detail


Peracetylated N-acetylmannosamine, a synthetic sugar molecule, efficiently rescues muscle phenotype and biochemical defects in mouse model of sialic acid-deficient myopathy.
MedLine Citation:
PMID:  22157763     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Distal myopathy with rimmed vacuoles/hereditary inclusion body myopathy (DMRV/hIBM), characterized by progressive muscle atrophy, weakness, and degeneration, is due to mutations in GNE, a gene encoding a bifunctional enzyme critical in sialic acid biosynthesis. In the DMRV/hIBM mouse model, which exhibits hyposialylation in various tissues in addition to muscle atrophy, weakness, and degeneration, we recently have demonstrated that the myopathic phenotype was prevented by oral administration of N-acetylneuraminic acid, N-acetylmannosamine, and sialyllactose, underscoring the crucial role of hyposialylation in the disease pathomechanism. The choice for the preferred molecule, however, was limited probably by the complex pharmacokinetics of sialic acids and the lack of biomarkers that could clearly show dose response. To address these issues, we screened several synthetic sugar compounds that could increase sialylation more remarkably and allow demonstration of measurable effects in the DMRV/hIBM mice. In this study, we found that tetra-O-acetylated N-acetylmannosamine increased cell sialylation most efficiently, and in vivo evaluation in DMRV/hIBM mice revealed a more dramatic, measurable effect and improvement in muscle phenotype, enabling us to establish analysis of protein biomarkers that can be used for assessing response to treatment. Our results provide a proof of concept in sialic acid-related molecular therapy with synthetic monosaccharides.
Authors:
May Christine V Malicdan; Satoru Noguchi; Tomoharu Tokutomi; Yu-ichi Goto; Ikuya Nonaka; Yukiko K Hayashi; Ichizo Nishino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-08
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-03-15     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2689-705     Citation Subset:  IM    
Affiliation:
Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Distal Myopathies / drug therapy*,  genetics,  metabolism,  pathology
Hexosamines / pharmacology*
Humans
Mice
Mice, Transgenic
Muscle, Skeletal / metabolism*,  pathology
N-Acetylneuraminic Acid / metabolism*
Chemical
Reg. No./Substance:
0/Hexosamines; 131-48-6/N-Acetylneuraminic Acid; 4773-29-9/N-acetylmannosamine
Comments/Corrections

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