Document Detail


Peptostreptococcus micros has a uniquely high capacity to form hydrogen sulfide from glutathione.
MedLine Citation:
PMID:  8510983     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There are high amounts of hydrogen sulfide in deep periodontal pockets. This volatile sulfur compound may be formed from L-cysteine, but only low levels of this amino acid can be expected to be present in periodontal pockets. Glutathione, L-gamma-glutamyl-L-cysteinylglycine, is in high concentration in most tissue cells, and this tripeptide may be more readily available as a source of hydrogen sulfide formation in the pockets. The ability of 37 different species of oral bacteria to utilize glutathione in hydrogen sulfide formation was studied. Of these species, only 2 species of Peptostreptococcus and 5 species of Fusobacterium formed high amounts of hydrogen sulfide from glutathione within 24 h. Since the initial rate of hydrogen sulfide formation was more than 5 times higher in Peptostreptococcus micros than in any of the other bacterial species, the kinetics of sulfide formation from glutathione by P. micros was further elucidated. The formation of sulfide followed quite closely hyperbolic Michaelis-Menten kinetics. The maximal initial rate of sulfide formation (Vmax) was 163 +/- 2 nmol sulfide per minute per milligram of cellular protein. Half maximal initial rate (Km) was obtained at 7.4 +/- 0.8 microM glutathione. The initial rate of sulfide formation from L-cysteine was much slower and was almost proportional to L-cysteine concentration. This difference in kinetics of sulfide formation between glutathione and L-cysteine strongly suggested that glutathione was actively transported into the cell, whereas the transport of L-cysteine was more or less controlled by diffusion. The sulfide formation from the dipeptide L-cysteinylglycine also followed quite closely hyperbolic Michaelis-Menten kinetics.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J Carlsson; J T Larsen; M B Edlund
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral microbiology and immunology     Volume:  8     ISSN:  0902-0055     ISO Abbreviation:  Oral Microbiol. Immunol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-07-15     Completed Date:  1993-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8707451     Medline TA:  Oral Microbiol Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  42-5     Citation Subset:  D    
Affiliation:
Department of Oral Microbiology, University of Umeå, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport, Active
Cysteine / metabolism
Dipeptides / metabolism
Glutathione / metabolism*
Hydrogen Sulfide / metabolism*
Peptostreptococcus / metabolism*
Chemical
Reg. No./Substance:
0/Dipeptides; 19246-18-5/cysteinylglycine; 52-90-4/Cysteine; 70-18-8/Glutathione; 7783-06-4/Hydrogen Sulfide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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