Document Detail

Peptidic sequence "HSEAETGPP" is recognized by the sera of pars planitis patients.
MedLine Citation:
PMID:  19480736     Owner:  NLM     Status:  MEDLINE    
PURPOSE: HLA class II, p-36 protein, heat shock protein and retinal antigens have been associated with pars planitis (PP), but their participation in the development of the disease are unknown. A search for new molecules related to PP is necessary. This work focused on the identification of peptides recognized by PP patient sera using the phage display method. METHODS: Sera of PP patients were used to isolate peptides fused to M13-phage pIII protein. The response of PP and healthy sera to peptides was determined by ELISA. PCR amplification and sequencing of peptide-encoding fragments from clones with high recognition by PP sera were used to characterize displayed peptides. RESULTS: One hundred clones were randomly selected from a phage display library after three panning rounds using serum proteins from a PP patient. The immunologic response level of 100 clones selected were determined with a major number of patients, it was found that one clone was recognized stronger in PP patients sera than in healthy sera (PP vs. healthy; P < 0.05). The peptide-encoding region of this clone was sequenced and translated. The peptide sequence corresponded to HSEAETGPP. An identical amino acid sequence to HSEAETGPP is found in the human proline-rich transmembrane protein 2 which has not been related with eye diseases. CONCLUSION: These results suggest that the peptide HSEAETGPP is associated with PP.
Jorge I Castañeda-Sánchez; Everardo Curiel-Quesada; Miguel Pedrosa-Seres; Mario E Cancino-Diaz; Sandra Rodríguez-Martínez; Juan C Cancino-Diaz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-01
Journal Detail:
Title:  Clinical and investigative medicine. Médecine clinique et experimentale     Volume:  32     ISSN:  1488-2353     ISO Abbreviation:  Clin Invest Med     Publication Date:  2009  
Date Detail:
Created Date:  2009-06-01     Completed Date:  2009-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804071     Medline TA:  Clin Invest Med     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  E206-11     Citation Subset:  IM    
Department of Biochemistry, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Mexico.
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MeSH Terms
Amino Acid Sequence
Enzyme-Linked Immunosorbent Assay
Pars Planitis / blood*,  immunology*
Peptide Library
Peptides / chemistry*,  genetics,  immunology*
Polymerase Chain Reaction
Reg. No./Substance:
0/Peptide Library; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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