| Peptides from Paracoccidioides brasiliensis GP43 inhibit macrophage functions and inflammatory response. | |
| | |
MedLine Citation:
|
PMID: 19026760 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by Paracoccidioides brasiliensis (Pb), a thermal dimorphic fungus. Its major antigen is a 43-kDa glycoprotein. Gp43 embodies different functions: it participates in evasion mechanisms during the installation of primary infection, stimulates granuloma-like formation in vitro and presents T-cell epitopes that induce protective response against the fungus. Here, we investigated epitopes from gp43 inhibitory of both, macrophage functions and inflammatory reaction. Different gp43 peptides, spanning the entire sequence of the molecule, were added to cultures of bone marrow-derived macrophages. After challenge with zymosan or Pb cells, phagocytic indexes were measured. Peptides expressed on the molecule surface were determined by graphic analysis using the Protean module; DNAstar Inc. Two peptides which decreased phagocytic index and were expressed at the surface of the molecule, P4 and P23, were selected for further studies. It was shown that both inhibited the release of NO by zymosan stimulated macrophages while enhanced release of H(2)O(2). The release of TNF-alpha in culture supernatants from in vitro phagocytic tests showed different response depending of P4 concentration (data not shown). In vivo assays with Mycobacterium bovis - bacillus Calmette-Guérin (BCG) or Pb cells demonstrated that these peptides presented non-specific and specific anti-inflammatory properties. |
| | |
Authors:
|
Adriana Y C Konno; Juliana T Maricato; Fabiana T C Konno; Mário Mariano; José Daniel Lopes |
Publication Detail:
|
Type: Journal Article Date: 2008-11-06 |
Journal Detail:
|
Title: Microbes and infection / Institut Pasteur Volume: 11 ISSN: 1286-4579 ISO Abbreviation: Microbes Infect. Publication Date: 2009 Jan |
Date Detail:
|
Created Date: 2009-01-26 Completed Date: 2009-05-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883508 Medline TA: Microbes Infect Country: France |
Other Details:
|
Languages: eng Pagination: 92-9 Citation Subset: IM |
Affiliation:
|
Universidade Federal de São Paulo - UNIFESP, Department of Microbiology, Immunology and Parasitology, Discipline of Immunology, São Paulo, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, Fungal / chemistry*, pharmacology Cells, Cultured Female Fungal Proteins / chemistry*, pharmacology Glycoproteins / chemistry*, pharmacology Inflammation / drug therapy Macrophages / drug effects*, immunology Male Mice Mice, Inbred BALB C Paracoccidioides / chemistry, immunology* Paracoccidioidomycosis / microbiology Peptides / chemical synthesis, chemistry, pharmacology* Phagocytosis / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/43 kDa protein, Paracoccidioides; 0/Antigens, Fungal; 0/Fungal Proteins; 0/Glycoproteins; 0/Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Establishment of long term cultures of neural stem cells from adult sea bass, Dicentrarchus labrax.
Next Document: Molecular pathology of age-related macular degeneration.