| Peptide nucleic acids (PNAs) antisense effect to bacterial growth and their application potentiality in biotechnology. | |
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MedLine Citation:
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PMID: 20135118 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peptide nucleic acids (PNAs) are nucleic acid analogs having attractive properties such as quiet stability against nucleases and proteases, and they form strong complexes with complementary strands of DNA or RNA. Because of this attractive nature, PNA is often used in antisense technology to inhibit gene expression and microbial cell growth with high specificity. Many bacterial antisense or antiribosomal studies using PNA oligomers have been reported so far, and parameters to design effective antisense PNAs and to improve PNA cell entry for efficient inhibition of bacterial growth have been presented. However, there are still several obstacles such as low cellular uptake of PNA while applying antisense PNAs to a complex microbial community. On overcoming these problems, the PNA antisense technique might become a very attractive tool not only for controlling the microbial growth but also for further elucidating microbial ecology in complex microbial consortia. Here, we summarize and present recent studies on the development of antimicrobial PNAs targeting mRNAs and rRNAs. In addition, the application potentiality of antisense techniques in nonclinical biotechnology fields is discussed. |
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Authors:
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Masashi Hatamoto; Akiyoshi Ohashi; Hiroyuki Imachi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2010-02-05 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 86 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 397-402 Citation Subset: IM |
Affiliation:
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Department of Social and Environmental Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima, Hiroshima, 739-8527, Japan. hatamoto@hiroshima-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology* Bacteria / drug effects* Biotechnology / methods* Growth Inhibitors / pharmacology* Oligonucleotides, Antisense / pharmacology* Peptide Nucleic Acids / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Growth Inhibitors; 0/Oligonucleotides, Antisense; 0/Peptide Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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