Document Detail

Peptide-mediated interference with baculovirus transduction.
MedLine Citation:
PMID:  18294718     Owner:  NLM     Status:  MEDLINE    
Baculovirus represents a multifunctional platform with potential for biomedical applications including disease therapies. The importance of F3, a tumor-homing peptide, in baculovirus transduction was previously recognized by the ability of F3 to augment viral binding and gene delivery to human cancer cells following display on the viral envelope. Here, F3 was utilized as a molecular tool to expand understanding of the poorly characterized baculovirus-mammalian cell interactions. Baculovirus-mediated transduction of HepG2 hepatocarcinoma cells was strongly inhibited by coincubating the virus with synthetic F3 or following incorporation of F3 into viral nucleocapsid by genetic engineering, the former suggesting direct interaction of the soluble peptide with the virus particles. Since internalization and nuclear accumulation of the virus were significantly inhibited or delayed, but the kinetics of viral binding, initial uptake, and endosomal release were unaffected, F3 likely interferes with cytoplasmic trafficking and subsequent nuclear transport of the virus. A polyclonal antibody raised against nucleolin, the internalizing receptor of F3, failed to inhibit cellular binding, but considerably reduced viral transduction efficiency, proposing the involvement of nucleolin in baculovirus entry. Together, these results render the F3 peptide a tool for elucidating the mechanism and molecular details conferring to baculovirus-mediated gene transduction in mammalian cells.
Anna R Mäkelä; Ale Närvänen; Christian Oker-Blom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-18
Journal Detail:
Title:  Journal of biotechnology     Volume:  134     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-03     Completed Date:  2008-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  20-32     Citation Subset:  IM    
NanoScience Center, Department of Biological and Environmental Science, PO Box 35, FIN-40014 University of Jyväskylä, Finland.
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MeSH Terms
Baculoviridae / genetics*
Biological Transport / drug effects
Blotting, Western
Cell Line
Cell Line, Tumor
Genetic Vectors / genetics*,  pharmacokinetics
Microscopy, Confocal
Peptides / chemical synthesis,  metabolism*,  pharmacology
Phosphoproteins / genetics,  metabolism
RNA-Binding Proteins / genetics,  metabolism
Transduction, Genetic
Reg. No./Substance:
0/Peptides; 0/Phosphoproteins; 0/RNA-Binding Proteins; 0/nucleolin

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