| Peptide-mediated delivery of nucleic acids into mammalian cells. | |
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MedLine Citation:
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PMID: 18604951 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Control of gene expression using RNA interference (RNAi) technology constitutes a method of choice for investigating gene function in mammalian cells. However, like most oligonucleotide-based strategies, the major limitation of interfering RNA is their poor cellular uptake due to low permeability of the cell membrane to nucleic acids. Several strategies have been developed to improve delivery of oligonucleotides both in cultured cells and in vivo. So far, there is no universal method for their delivery, as they all present several limitations. Peptide-based strategies have been demonstrated to improve the cellular uptake of nucleic acids both in cultured cell and in vivo. This chapter describes a new peptide-based gene delivery system, MPG, which forms stable noncovalent complexes with oligonucleotides and promotes their delivery into a large panel of cell lines without the need for prior chemical covalent coupling. Protocols are described for both adherent and suspension cell lines. |
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Authors:
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Sébastien Deshayes; Federica Simeoni; May C Morris; Gilles Divita; Frédéric Heitz |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 386 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2008-07-07 Completed Date: 2008-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 299-308 Citation Subset: IM |
Affiliation:
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Center de Recherches de Biochimie Macromoleculaire, Department of Molecular Biophysics & Therapeutics, Montpellier, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cell Line DNA-Binding Proteins / chemistry Drug Carriers / chemistry* Drug Delivery Systems Hela Cells Humans Jurkat Cells Lipids / chemistry Macromolecular Substances / chemistry Mice Molecular Biology / methods Molecular Sequence Data Nucleic Acids / administration & dosage*, genetics Peptides / chemistry* RNA Interference RNA, Small Interfering / administration & dosage, genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Drug Carriers; 0/Lipids; 0/MPG peptide; 0/Macromolecular Substances; 0/Nucleic Acids; 0/Peptides; 0/RNA, Small Interfering |
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