| Peptide display on filamentous phage capsids. A new powerful tool to study protein-ligand interaction. | |
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MedLine Citation:
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PMID: 1639197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peptides can be displayed on the surface of filamentous bacteriophages by fusion to phage coat proteins. It was recently shown that vast (10(8)) collections of phages, each exposing a variant of the original peptide, can be constructed and utilized as a general source of peptide ligands. By panning these libraries on a target molecule linked to a solid support it is possible to select, out of the hundreds of millions of clones, those few phages that display a peptide that binds the target molecule. Searching these libraries is a powerful tool to be applied in many areas of fundamental and applied biology. |
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Authors:
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G Cesareni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: FEBS letters Volume: 307 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1992 Jul |
Date Detail:
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Created Date: 1992-09-02 Completed Date: 1992-09-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 66-70 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Tor Vergata, Rome, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriophages
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genetics,
metabolism* Capsid / genetics, metabolism* Genomic Library Ligands Recombinant Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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