Document Detail

Peptide-YY is released by the intestinal cell line STC-1.
MedLine Citation:
PMID:  19323755     Owner:  NLM     Status:  MEDLINE    
Dietary modulation of the response of gut satiety hormones, which partly regulate food intake, provides a promising treatment for overweight and obesity. Gut-derived cell lines such as STC-1 are widely used to investigate these hormonal responses to nutrients. To date, no peptide-YY (PYY) secreting cell line has been identified. The aim of this study was to investigate whether STC-1 cells are able to secrete PYY and if so, whether dietary compounds can modulate PYY secretion. The effects of fatty acid types C4:0, C12:0, C14:0, C16:0, and C18:0 on PYY release were investigated by measuring PYY in the supernatant after 30, 60, 90, and 120 min of incubation, respectively, using RIA assays. The STC-1 cells were able to secrete PYY in a time-dependent manner. It was shown that after 30 min, C4:0, C12:0, C16:0, and C18:0 caused increased PYY levels compared to the control. At time points 60 and 90 min, C4:0 and C18:0 induced elevated PYY levels compared to the control. After 120 min, C4:0, C14:0, and C18:0 caused elevated levels compared to the control. We are the first to show that the STC-1 cells are also able to secrete PYY next to cholecystokinin (CCK) and glucagon-like peptide 1 (GLP-1). Addition of fatty acids resulted in increased levels of PYY, which is consistent with the literature describing human studies. We conclude that the STC-1 cell line provides an appropriate cell line for screening the effects of ingredients on the release of the satiety-related gut hormones CCK, GLP-1, and PYY.
M C P Geraedts; F J Troost; W H M Saris
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food science     Volume:  74     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-27     Completed Date:  2009-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H79-82     Citation Subset:  IM    
Dept of Human Biology, Div of Gastroenterology and Hepatology, All Nutrition and Toxicology Research Inst Maastricht, Maastricht Univ, Maastricht, The Netherlands.
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MeSH Terms
Cell Line / drug effects,  secretion*
Dietary Fats / pharmacology*
Fatty Acids, Nonesterified / pharmacology*
Intestines / metabolism*
Peptide YY / secretion*
Reg. No./Substance:
0/Dietary Fats; 0/Fatty Acids, Nonesterified; 106388-42-5/Peptide YY

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