| Peptide sugar mimetics prevent HIV type 1 replication in peripheral blood mononuclear cells in the presence of HIV-positive antiserum. | |
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MedLine Citation:
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PMID: 20156098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells of the immune system express a number of receptors that bind carbohydrate ligands. We questioned whether peptide mimetics of these ligands will activate phagocytic cells and thereby enhance an antiviral response. Short peptide sequences were identified by computational modeling of docking to glycan-specific lectins, selected as receptor analogs, and incorporated into quadravalent structures by peptide synthesis. A peptide with the sequence HPSLK bound to several lectins specific for monosaccharides and to lectins specific for Neu5Ac-Gal-containing complex glycans, whereas a longer sequence, NPSHPLSG, bound only lectins specific for the more complex glycans. In cultures of peripheral blood mononuclear cells (PBMCs) these peptides stimulated phagocytosis of opsonized microspheres. The peptides inhibited replication of HIV-1 in PBMC cultures by 20-80% at concentrations between 1 nM and 1 muM but inhibited replication 100% in the presence of diluted HIV-positive antiserum that alone inhibited replication by 30%. HPSLK caused about 50% loss of viability of cells at 1 mM, a concentration 10(6)-fold higher than an effective inhibitory concentration, but no toxicity was observed with NPSHPLSG. These results demonstrated that peptidomimetics of glycan ligands of cellular receptors are effective in activating phagocytosis, which may be a factor in providing complete inhibition of HIV-1 replication in vitro. |
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Authors:
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Laura L Eggink; Maria Salas; Carl V Hanson; J Kenneth Hoober |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: AIDS research and human retroviruses Volume: 26 ISSN: 1931-8405 ISO Abbreviation: AIDS Res. Hum. Retroviruses Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-16 Completed Date: 2010-04-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709376 Medline TA: AIDS Res Hum Retroviruses Country: United States |
Other Details:
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Languages: eng Pagination: 149-60 Citation Subset: IM; X |
Affiliation:
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Susavion Biosciences, Inc., Tempe, AZ 85281, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-HIV Agents
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pharmacology*,
toxicity Biomimetics* Cell Survival / drug effects Cells, Cultured HIV-1 / drug effects* Humans Leukocytes, Mononuclear / virology* Peptides / pharmacology*, toxicity Virus Replication / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Anti-HIV Agents; 0/Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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