Document Detail


Pepsinogens and pepsins from mandarin fish (Siniperca chuatsi).
MedLine Citation:
PMID:  18543931     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four pepsinogens (PG-I, PG-II, PG-III(a), and PG-III(b)) were highly purified from the stomach of the freshwater fish mandarin fish (Siniperca chuatsi) by ammonium sulfate fractionation, anion exchange, and gel filtration. The molecular masses of the four purified PGs were 36, 35, 38, and 35 kDa, respectively. All the pepsinogens converted into their active form pepsins within a few minutes under pH 2.0. The optimum pH and temperature of the four enzymes were 3.0-3.5 and 45-50 degrees C, using hemoglobin as a substrate. The N-terminal amino acid sequences of PG-I and PG-II were determined to the 12th and 17th amino acid residues, respectively. Western blot analysis using antisea bream polyclonal antibodies cross reacted with PG-I, PG-II, and PG-III(b) while no cross reaction with PG-III(a) was detected, suggesting the diversity of pepsinogens in fish.
Authors:
Qiong Zhou; Guang-Ming Liu; Yuan-Yuan Huang; Ling Weng; Kenji Hara; Wen-Jin Su; Min-Jie Cao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-11
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-03     Completed Date:  2008-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5401-6     Citation Subset:  IM    
Affiliation:
College of Biological Engineering, The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Jimei, Xiamen, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
China
Chromatography
Electrophoresis, Polyacrylamide Gel
Enzyme Stability
Fresh Water
Molecular Sequence Data
Molecular Weight
Pepsin A / chemistry*,  isolation & purification,  metabolism
Pepsinogens / chemistry*,  isolation & purification,  metabolism
Perciformes / genetics,  metabolism*
Sequence Alignment
Stomach / enzymology
Chemical
Reg. No./Substance:
0/Pepsinogens; EC 3.4.23.1/Pepsin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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