Document Detail


Pepsinogen and H,K-ATPase mediate acid secretion in gastric glands of Triturus carnifex (Amphibia, Caudata).
MedLine Citation:
PMID:  15878191     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gastric glands of Triturus carnifex (Amphibia, Caudata) have been examined by histochemical and immunohistochemical methods with particular regard to hydrochloric acid and pepsinogen secretion. Fundic glands consist of mucous neck cells, endocrine cells and oxynticopeptic cells producing both pepsinogen and hydrochloric acid. The neck cells showed an unexpected distribution pattern which was only observed in the oral fundus, and produced neutral mucins with glycosidic residues of GalNAc and Gal beta1,3GalNAc, and in this respect they differ from the neck cells of anuran amphibians. The secretion of pepsinogen and hydrochloric acid as demonstrated by immunolabelling with anti-H,K-ATPase and with anti-pepsinogen, respectively, seems not to vary significantly along the longitudinal axis of the stomach. The mechanism of gastric acid secretion seems to be mediated by an ATPase, having similar features to the mammalian gastric H,K-ATPase, and is localised in the luminal membrane and in the subapical cytoplasm of the oxynticopeptic cells. Unusually, the same cytoplasmic areas revealed binding specificity for the winged pea lectin (WPA) from Lotus tetragonolobus, even after beta elimination, indicating the presence of fucosyl residues in N-linked oligosaccharidic chains in glycoproteins of beta-H,K-ATPase subunits.
Authors:
Giuseppa Esterina Liquori; Sara Zizza; Maria Mastrodonato; Giovanni Scillitani; Giuseppe Calamita; Domenico Ferri
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta histochemica     Volume:  107     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-06-13     Completed Date:  2006-03-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  133-41     Citation Subset:  IM    
Affiliation:
Department of Zoology, Laboratory of Histology and Comparative Anatomy, University of Bari, Via Orabona 4, 70125 Bari, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Gastric Acid / secretion*
Gastric Mucosa / drug effects,  enzymology*,  metabolism,  secretion*
H(+)-K(+)-Exchanging ATPase / metabolism*
Immunohistochemistry
Pepsinogen A / metabolism*
Plant Lectins / pharmacology
Triturus / anatomy & histology,  metabolism*
Chemical
Reg. No./Substance:
0/Plant Lectins; 9001-10-9/Pepsinogen A; EC 3.6.1.10/H(+)-K(+)-Exchanging ATPase

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