Document Detail


Pepsinogen C and pepsin C from gastric mucosa of Japanese monkey. Purification and characterization.
MedLine Citation:
PMID:  12157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new pepsinogen component, pepsinogen C, was purified from the gastric mucosa of Japanese monkey. The chromatographic behavior of this component on DE-32 cellulose was coincident with that of pepsinogen III-2 previously reported (1), and final purification was performed by large-scale polyacrylamide disc gel electrophoresis. The molecular weight was 35,000 as determined by gel filtration. The ratios of glutamic acid to aspartic acid and of leucine to isoleucine were higher than those of other Japanese monkey pepsinogens. The activated form, pepsin C, had a molecular weight of 27,000 and contained a large number of glutamic acid residues. The optimal pH for hemoglobin digestion was 3.0. Pepsin C could scarcely hydrolyze the synthetic substrate, N-acetyl-L-phenylalanyl-3, 5-diiodo-L-tyrosine (APDT). 1, 2-Epoxy-3-(p-nitrophenoxy)propane (EPNP), p-bromophenacyl bromide, and diazoacetyl-DL-norleucine methyl ester (DAN) inhibited pepsin C [EC 3.4.23.3] in the same way as pepsin III-3 of Japanese monkey. The susceptibility to pepstatin of pepsin C was lower than that of pepsin III-3, and 500 times more pepstatin was required for the same inhibitory effect. The classification and nomenclature of Japanese monkey pepsinogens and pepsins are discussed.
Authors:
T Kageyama; K Takahashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  80     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-02-26     Completed Date:  1977-02-26     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  983-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Azo Compounds / pharmacology
Epoxy Compounds / pharmacology
Gastric Mucosa / enzymology*
Haplorhini
Hydrogen-Ion Concentration
Macaca
Molecular Weight
Nitrophenols / pharmacology
Norleucine / analogs & derivatives,  pharmacology
Pepsin A / metabolism*
Pepsinogens / isolation & purification,  metabolism*
Pepstatins / pharmacology
Phenylacetates / pharmacology
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Azo Compounds; 0/Epoxy Compounds; 0/Nitrophenols; 0/Pepsinogens; 0/Pepstatins; 0/Phenylacetates; 5157-09-5/Norleucine; EC 3.4.23.1/Pepsin A

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