Document Detail

Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity.
MedLine Citation:
PMID:  11337271     Owner:  NLM     Status:  MEDLINE    
Oral squamous carcinoma cell line SSCKN cells were shown to be highly sensitive to bleomycin, whereas SCCTF cells were minimally sensitive to this reagent. To determine whether the anticancer drug resistance to oral squamous carcinoma cells could be related to the degree of the drug-induced apoptosis, we examined the effects of peplomycin on induction of apoptosis in these cells. After reaching subconfluence, SCCKN and SCCTF cells were exposed to various concentrations of peplomycin. Peplomycin caused cytotoxicity in both SCCKN and SCCTF cells in a dose-dependent fashion with the maximal effect at concentrations of 1 and 10 microM, respectively, as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, we observed marked nuclear condensation and fragmentation of chromatin in SCCKN cells treated with 1 microM peplomycin. However, SCCTF cells treated with 1 microM peplomycin showed neither nuclear condensation nor fragmentation. DNA ladder formation was also detected in both cell lines by treatment with peplomycin. The induced DNA ladder formation in SCCKN and SCCTF cells was dose-dependent, with the maximal effect at concentrations of 5 and 50 microM, respectively. Bleomycin also induced DNA ladder formation in SCCKN and SCCTF cells with different sensitivities. Mitomycin C induced DNA laddering in both SCCKN and SCCTF cells; however, the intensity of DNA ladder formation was almost the same in both cell lines. The present results indicate that peplomycin-induced apoptosis in oral squamous carcinoma cell lines depends on the sensitivity of these cells to bleomycin.
H Okamura; H Morimoto; T Haneji
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral oncology     Volume:  37     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-04     Completed Date:  2001-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  379-85     Citation Subset:  IM    
Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 3-18-15,3 Kuramoto, 770-8504, Tokushima, Japan.
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MeSH Terms
Antibiotics, Antineoplastic / therapeutic use*
Apoptosis / physiology*
Bleomycin / therapeutic use*
Carcinoma, Squamous Cell / drug therapy*,  pathology
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Drug Resistance, Neoplasm
Microscopy, Phase-Contrast
Mitomycin / therapeutic use
Mouth Neoplasms / drug therapy*,  pathology
Peplomycin / therapeutic use*
Treatment Outcome
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 11056-06-7/Bleomycin; 50-07-7/Mitomycin; 68247-85-8/Peplomycin

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