Document Detail

Peony glycosides protect against corticosterone-induced neurotoxicity in PC12 cells.
MedLine Citation:
PMID:  19214737     Owner:  NLM     Status:  MEDLINE    
Preclinical and clinical investigations have shown the involvement of dysregulation of hypothalamic-pituitary-adrenal (HPA) axis in the pathogenesis of depression. Hypercortisolemia and the associated hippocampal atrophy were observed in patients with depression, which could be ameliorated by the treatment with antidepressants. Therefore, neuroprotection has been proposed to be one of the acting mechanisms of antidepressant. Previous studies in our laboratory have demonstrated the antidepressant-like activity of total glycosides of peony (TGP) in mice. This study aimed to examine the effect of TGP treatment on corticosterone-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. Treating the cells with corticosterone at 200 muM for 48 h caused apoptotic cell death. The cytotoxicity was associated with the activation of caspase-3 activity and the decrease in the mRNA ratio of bcl-2 to bax. TPG treatment at increasing doses (1-10 mg/l) protected against the corticosterone-induced toxicity in PC12 cells in a dose-dependent manner. The cytoprotection afforded by TGP treatment was associated with the inhibition of caspase-3 activity and the up-regulation of bcl-2/bax mRNA ratio. The anti-apoptotic effect of TGP is therefore likely mediated by the suppression of the mitochondrial pathway leading to apoptosis.
Qing-Qiu Mao; Siu-Po Ip; Kam-Ming Ko; Sam-Hip Tsai; Ming Zhao; Chun-Tao Che
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-12
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  29     ISSN:  1573-6830     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-15     Completed Date:  2009-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  643-7     Citation Subset:  IM    
School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
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MeSH Terms
Caspase 3 / metabolism
Cell Death / drug effects
Cell Survival / drug effects
Corticosterone / toxicity*
DNA Fragmentation / drug effects
Gene Expression Regulation / drug effects
Glycosides / pharmacology*
Neurons / cytology*,  drug effects*
Neuroprotective Agents / pharmacology*
PC12 Cells
Paeonia / chemistry*
RNA, Messenger / genetics,  metabolism
bcl-2-Associated X Protein / genetics,  metabolism
Reg. No./Substance:
0/Glycosides; 0/Neuroprotective Agents; 0/RNA, Messenger; 0/bcl-2-Associated X Protein; 50-22-6/Corticosterone; EC 3.4.22.-/Caspase 3

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