| Pentylenetetrazole-induced convulsions affect cellular and molecular parameters of the mechanism of action of triiodothyronine in adult rat brain. | |
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MedLine Citation:
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PMID: 15829259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the current study was to elucidate whether the response of the adult rat brain to thyroid hormones is affected by the intensity of neuronal activity. For this purpose, the kinetic characteristics of nuclear T3 binding, the relative expression of thyroid hormone receptor (TR) isoforms and the synaptosomal content of thyroid hormones in adult rat brain were examined after administration of a single convulsion dose of pentylenetetrazole (PTZ). Experiments in adult Wistar rats revealed an increase (33%) of the density of specific T3 nuclear receptors in cerebral hemispheres 4h after PTZ-induced seizures while no changes were observed in the dissociation constant. The relative expression of the T3-binding isoforms of TRs was not affected, while there was a gradual decrease of the relative expression of the TR alpha2 variant (non-T3 binding isoform). The above changes were coupled with an increase of the synaptosomal T3 levels during the epileptic seizures. Our study revealed inversely proportional changes between the nuclear T3 binding sites and the TR alpha2 mRNA levels 4 h after PTZ-induced seizures, suggesting that the regulation of the expression of the non-T3 binding variant of TRs determines the nuclear T3 binding sites in adult rat brain, while the synaptosomal T3 levels could play a novel functional role in the signaling from the synapse to the nucleus. |
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Authors:
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Stamatis Bolaris; Caterina Constantinou; Theony Valcana; Marigoula Margarity |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuropharmacology Volume: 48 ISSN: 0028-3908 ISO Abbreviation: Neuropharmacology Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-04-14 Completed Date: 2005-06-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0236217 Medline TA: Neuropharmacology Country: England |
Other Details:
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Languages: eng Pagination: 894-902 Citation Subset: IM |
Affiliation:
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Department of Human Reproduction, Helena Venizelou Hospital, 2 Venizelou Sqr., 11521 Athens, Greece. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern / methods Cerebral Cortex / cytology, drug effects*, metabolism Disease Models, Animal Drug Interactions Female Gene Expression Regulation / drug effects Iodine Isotopes / pharmacology Male Pentylenetetrazole / pharmacology* Protein Binding / drug effects, physiology RNA, Messenger / metabolism Rats Rats, Wistar Seizures / chemically induced, metabolism* Synaptosomes / drug effects, metabolism Time Factors Triiodothyronine / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Iodine Isotopes; 0/RNA, Messenger; 54-95-5/Pentylenetetrazole; 6893-02-3/Triiodothyronine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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