| Pentoxifylline reduces leukocyte retention in the coronary microcirculation early in reperfusion following ischemia. | |
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MedLine Citation:
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PMID: 8923149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using direct visualization techniques, we recently confirmed earlier histologic studies that leukocytes accumulate primarily in the coronary capillaries of ischemic hearts during early reperfusion. The purpose of this study was to determine if pentoxifylline (PTX) would reduce leukocyte trapping in postischemic hearts. Isolated rat hearts were subjected to 30 min of 37 degrees C, no-flow ischemia. Hearts were initially reperfused with diluted whole blood containing fluorescent leukocytes. At 5, (R5), 20, and 35 min of reperfusion, the deposition of leukocytes in the coronary capillaries and venules was observed directly using intravital fluorescence microscopy. Three groups were studied: a non-ischemic control group (group I) and postischemic groups reperfused with diluted whole blood treated with vehicle group II or PTX (5 mM; group III). Postischemic reperfusion with unactivated blood caused a significant trapping of leukocytes in coronary capillaries throughout reperfusion (R5, group I = 2.0 +/- 0.3 vs. group II = 5.7 +/- 0.6 leukocytes/capillary field, p < 0.05). The addition of PTX reduced capillary leukocyte trapping below control values throughout reperfusion (R5, group III = 1.6 +/- 0.2 leukocytes/capillary field, p < 0.05). At R5, there was no statistically significant difference in leukocyte accumulation in venules for all groups (group I = 1.5 +/- 0.6, group II = 3.2 +/- 0.4, group III = 3.3 +/- 0.4 leukocytes/100 microns venule). During the reperfusion period, leukocyte persistence in the capillaries of postischemic hearts (36%) was greater than in the venules (13%). These data indicate that early in reperfusion after myocardial ischemia, leukocyte trapping occurs primarily in the coronary capillaries. PTX reduced early leukocyte trapping in the capillaries. The results also demonstrate that during reperfusion, the mechanisms affecting capillary retention are more persistent than those in the venule. These findings suggest that attempts to attenuate the damaging potential of early leukostasis in capillaries consider the biophysical properties of the leukocyte. |
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Authors:
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L S Ritter; D S Wilson; S K Williams; J G Copeland; P F McDonagh |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation Volume: 16 ISSN: 0167-6865 ISO Abbreviation: Int J Microcirc Clin Exp Publication Date: 1996 Jul-Aug |
Date Detail:
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Created Date: 1997-02-27 Completed Date: 1997-02-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8400122 Medline TA: Int J Microcirc Clin Exp Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 170-9 Citation Subset: IM |
Affiliation:
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Department of Surgery, College of Medicine, University of Arizona, Tucson 85724-5084, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion / drug effects Coronary Vessels / drug effects*, pathology Leukocytes / drug effects, pathology* Male Microcirculation / drug effects Microscopy, Fluorescence Myocardial Ischemia / drug therapy, pathology* Pentoxifylline / pharmacology* Rats Rats, Sprague-Dawley Reperfusion Vascular Resistance Vasodilator Agents / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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07249//PHS HHS; HL 49230/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Vasodilator Agents; 6493-05-6/Pentoxifylline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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