Document Detail

Pentoxifylline reduces indirect lung injury of fresh water drowning in canis.
MedLine Citation:
PMID:  16236273     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Previous studies have suggested that pentoxifylline (PTX) exerts multiple beneficial effects on the inflammatory cascade, particularly on the function of neutrophils. We investigated whether continuous infusion of PTX could reduce indirect lung injury (ILI) caused by fresh water drowning and if so, what is the possible molecular mechanism. METHODS: Twenty 4 male canis were divided randomly into control group, fresh water drowning group (right lung), drowning treated with PTX group and PTX alone group. At different time points after drowning, the changes of hemodynamic parameters and wet/dry weight of indirect lung (left lung) tissues were compared among these 4 groups. Other measures included lung histopathology, PMN infiltration assessed by immune staining, CD11b, ICAM-1 mRNA and TNF-alphamRNA in left lung detected by RT-PCR. NF-kappaB activation in blood neutrophils and lungs were measured with electrophoretic mobility shift assay (EMSA). RESULTS: Animals treated with PTX showed a significant reduction in lung injury. PTX suppressed drowning-induced ICAM-1 and TNF-alphamRNA elevation and inhibited NF-kappaB activation in blood neutrophils and lungs. CONCLUSIONS: Continuous infusion of PTX reduces ILI caused by fresh water drowning. PTX decreases expression of ICAM-1 and TNF-alpha, possibly via inhibition of NF-kappaB.
Qing Ji; Lidong Zhang; Liqing Wang; Hongbin Jia; Jianguo Xu
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-10-19
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  365     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-06     Completed Date:  2006-06-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  221-9     Citation Subset:  IM    
Department of Anesthesiology, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, P.R. China.
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MeSH Terms
Antigens, CD11b / metabolism
Base Sequence
DNA Primers
Electrophoretic Mobility Shift Assay
Intercellular Adhesion Molecule-1 / genetics
Lung / drug effects*,  metabolism,  pathology
Lung Injury
NF-kappa B / metabolism
Neutrophils / cytology
Organ Size
Pentoxifylline / pharmacology*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha / genetics
Reg. No./Substance:
0/Antigens, CD11b; 0/DNA Primers; 0/NF-kappa B; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 126547-89-5/Intercellular Adhesion Molecule-1; 6493-05-6/Pentoxifylline

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