| Pentosan polysulfate protects brain endothelial cells against bacterial lipopolysaccharide-induced damages. | |
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MedLine Citation:
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PMID: 16997427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peripheral inflammation can aggravate local brain inflammation and neuronal death. The blood-brain barrier (BBB) is a key player in the event. On a relevant in vitro model of primary rat brain endothelial cells co-cultured with primary rat astroglia cells lipopolysaccharide (LPS)-induced changes in several BBB functions have been investigated. LPS-treatment resulted in a dose- and time-dependent decrease in the integrity of endothelial monolayers: transendothelial electrical resistance dropped, while flux of permeability markers fluorescein and albumin significantly increased. Immunostaining for junctional proteins ZO-1, claudin-5 and beta-catenin was significantly weaker in LPS-treated endothelial cells than in control monolayers. LPS also reduced the intensity and changed the pattern of ZO-1 immunostaining in freshly isolated rat brain microvessels. The activity of P-glycoprotein, an important efflux pump at the BBB, was also inhibited by LPS. At the same time production of reactive oxygen species and nitric oxide was increased in brain endothelial cells treated with LPS. Pentosan polysulfate, a polyanionic polysaccharide could reduce the deleterious effects of LPS on BBB permeability, and P-glycoprotein activity. LPS-stimulated increase in the production of reactive oxygen species and nitric oxide was also decreased by pentosan treatment. The protective effect of pentosan for brain endothelium can be of therapeutical significance in bacterial infections affecting the BBB. |
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Authors:
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Szilvia Veszelka; Mária Pásztói; Attila E Farkas; István Krizbai; Thi Khue Dung Ngo; Masami Niwa; Csongor S Abrahám; Mária A Deli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-25 |
Journal Detail:
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Title: Neurochemistry international Volume: 50 ISSN: 0197-0186 ISO Abbreviation: Neurochem. Int. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-12 Completed Date: 2007-03-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006959 Medline TA: Neurochem Int Country: England |
Other Details:
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Languages: eng Pagination: 219-28 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt 62, H-6726 Szeged, Hungary. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood-Brain Barrier Brain / blood supply, drug effects* Endothelium, Vascular / cytology, drug effects* Lipopolysaccharides / pharmacology* Membrane Potentials / drug effects P-Glycoprotein / metabolism Pentosan Sulfuric Polyester / pharmacokinetics, pharmacology* Rats |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/P-Glycoprotein; 37300-21-3/Pentosan Sulfuric Polyester |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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