Document Detail


Pentalysine-grafted ROMP polymers for DNA complexation and delivery.
MedLine Citation:
PMID:  18665641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amphiphilic graft polymers, containing oligolysine groups pendent to a hydrophobic polycyclooctene backbone, were used to form polyplexes with plasmid DNA pZsGreen1-N1. These poly(cyclooctene- graft-pentalysine) structures were found to be effective transfection reagents for COS-1 and HeLa cells. In the case of polymer 1e (average degree of polymerization of 206), protein expression levels 48 h post-transfection were found to be comparable to, or better than, commercial transfection reagents jetPEI and SuperFect. With HeLa cells, GFP expression levels were better than Lipofectamine 2000. Of particular interest was the excellent cell viability seen in experiments with polyplexes formed from the pentalysine-grafted polymers. In the example of the highest molecular weight graft copolymer, polymer 1e, cell viability relative to untreated cells was 99% with COS-1 cells and 92% with HeLa cells in contrast to the commercial reagents, which gave 67-80% with COS-1 cells and 17-52% with HeLa cells. The effectiveness of these polyolefin- graft-pentalysine structures as DNA delivery vehicles is attributed to their amphiphilic nature and branched architecture.
Authors:
Rebecca B Breitenkamp; Todd Emrick
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-07-30
Journal Detail:
Title:  Biomacromolecules     Volume:  9     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-08     Completed Date:  2009-01-26     Revised Date:  2014-11-02    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2495-500     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Cations / chemical synthesis,  chemistry
Cell Survival / drug effects
Cercopithecus aethiops
DNA / chemistry*,  metabolism*
Drug Delivery Systems*
Drug Design
Gene Transfer Techniques*
Green Fluorescent Proteins / biosynthesis,  drug effects
HeLa Cells
Humans
Molecular Structure
Molecular Weight
Polyethylene Glycols / chemistry
Polymers / chemistry*
Stereoisomerism
Structure-Activity Relationship
Grant Support
ID/Acronym/Agency:
T32 GM008515/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cations; 0/Polyethylene Glycols; 0/Polymers; 0/poly(cyclooctene-graft-pentalysine); 147336-22-9/Green Fluorescent Proteins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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