Document Detail


Penta-O-galloyl-β-d-glucose attenuates cisplatin-induced nephrotoxicity via reactive oxygen species reduction in renal epithelial cells and enhances antitumor activity in Caki-2 renal cancer cells.
MedLine Citation:
PMID:  22172427     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cisplatin shows limited therapeutic efficacy due to serious side effects such as nephrotoxicity and hepatotoxicity. In the present study, we demonstrate that 1,2,3,4,6-penta-O-galloyl-β-d-glucose (PGG) has protective effects against cisplatin-induced cytotoxicity and apoptosis in normal human primary renal epithelial cells (HRCs) while showing synergistic effect against cisplatin-induced cell death in human Caki-2 renal cancer cells. PGG significantly blocked cisplatin-mediated cytotoxicity and reduced cisplatin induced sub-G1 accumulation in HRCs. Consistently, PGG reduced the number of apoptotic cell populations by TdT-mediated dUTP nick end labeling (TUNEL) and Live/Dead assays in cisplatin-treated HRCs. Furthermore, PGG suppressed PARP cleavage and caspase-3 activation, cytochrome c release, upregulation of bax and p53 in cisplatin-treated HRCs. Moreover, PGG attenuated reactive oxygen species (ROS) production mediated by cisplatin treatment, suggesting that PGG prevented cisplatin-induced apoptosis by inhibiting ROS generation in HRCs. Notably, PGG significantly enhanced cytotoxicity and PARP cleavage in cisplatin treated Caki-2 renal cancer cells. Combination Index (CI) revealed synergism between PGG and cisplatin in Caki-2 cells. Taken together, our findings suggest the dual effects of PGG as a protective supplement against cisplatin-induced toxicity in normal renal cells and a combination chemotherapeutic drug with cisplatin in renal cancer cells.
Authors:
Ho-Geon Ryu; Soo-Jin Jeong; Hee-Young Kwon; Hyo-Jung Lee; Eun-Ok Lee; Min-Ho Lee; Seung Hoon Choi; Kyoo Seok Ahn; Sung-Hoon Kim
Related Documents :
12894557 - Flow cytometric evaluation of apoptosis and cell viability as a criterion of anti-tumou...
16923337 - Cytotoxic and apoptotic effects of prenylflavonoid artonin b in human acute lymphoblast...
17497287 - The effect of hbx gene on the apoptosis of hepatic cells.
14982037 - Inhibitory effect of heat shock protein 70 on apoptosis induced by photodynamic therapy...
21055947 - Wnt5a/jnk and fgf/mapk pathways regulate the cellular events shaping the vertebrate lim...
11761447 - Auricular acupuncture increases cell proliferation in the dentate gyrus of sprague-dawl...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-7
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  -     ISSN:  1879-3177     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
College of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, South Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Investigation on the genotoxicity of different sizes of gold nanoparticles administered to the lungs...
Next Document:  Consumption of gold kiwifruit reduces severity and duration of selected upper respiratory tract infe...