Document Detail

Penile enhancement using autologous tissue engineering with biodegradable scaffold: a clinical and histomorphometric study.
MedLine Citation:
PMID:  20796201     Owner:  NLM     Status:  In-Process    
INTRODUCTION: Autologous tissue engineering with biodegradable scaffolds is a new treatment option for real penile girth enhancement.
AIM: The aim of this article is to evaluate tissue remodeling after penile girth enhancement using this technique.
METHODS: Between June 2005 and May 2007, a group of 12 patients underwent repeated penile widening using biodegradable scaffolds enriched with expanded autologous scrotal dartos cells. Clinical monitoring was parallel to histological investigation of tissue remodeling. During second surgical procedure, biopsies were obtained 10-14 months after first surgery (mean 12 months, N=6) and compared with those obtained after 22-24 months (mean 23 months, N=6), and control biopsies from patients who underwent circumcision (N=5). Blind evaluation of histomorphometrical and immunohistochemical finding was performed in paraffin sections.
MAIN OUTCOME MEASUREMENTS: Penile girth gain in a flaccid state ranged between 1.5 and 3.8 cm (mean 2.1 ± 0.28 cm) and in full erection between 1.2 and 4 cm (mean 1.9 ± 0.28 cm). Patients' satisfaction, defined by a questionnaire, was good (25%) and very good (75%).
RESULTS: In biopsies obtained 10-14 months after first surgery, highly vascularized loose tissue with collagen deposition associated with small foci of mild chronic and granulomatous inflammation surrounding residual amorphous material was observed. Fibroblast-like hyperplasia and small vessel neoangiogenesis occurred intimately associated with the progressive growth of vascular-like structures from accumulation of CD34 and alpha-smooth muscle actin-positive cells surrounding residual scaffold-like amorphous material. Capillary neoangiogenesis occurred inside residual amorphous material. In biopsies obtained after 22-24 months, inflammation almost disappeared and tissue closely resembled that of the dartos fascia of control group.
CONCLUSIONS: Autologous tissue engineering using expanded scrotal dartos cells with biodegradable scaffolds is a new and promising method for penile widening that generates progressive accumulation of stable collagen-rich, highly vascularized tissue matrix that closely resemble deep dartos fascia.
Sava V Perovic; Salvatore Sansalone; Rados Djinovic; Amedeo Ferlosio; Giuseppe Vespasiani; Augusto Orlandi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of sexual medicine     Volume:  7     ISSN:  1743-6109     ISO Abbreviation:  J Sex Med     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101230693     Medline TA:  J Sex Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3206-15     Citation Subset:  IM    
Copyright Information:
© 2009 International Society for Sexual Medicine.
Serbian Academy of Science and Arts, School of Medicine-University of Belgrade, Belgrade, Serbia.
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