Document Detail

Penetration of zona-free hamster oocytes using human sperm aspirated from the epididymis of men with congenital absence of the vas deferens: comparison with human in vitro fertilization.
MedLine Citation:
PMID:  1426349     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To assess the ability of sperm aspirated from the epididymis of men with congenital absence of the vas deferens to penetrate zona-free hamster oocytes. To directly compare the performance of human epididymal sperm in the zona-free hamster oocyte sperm penetration assay (SPA) with the results of human in vitro fertilization (IVF). DESIGN: Sperm penetration assay was carried out with epididymal sperm retrieved microsurgically, and with ejaculated sperm obtained from fertile donors (internal controls). For direct comparison, SPA was performed with the same epididymal sperm sample used for IVF. PATIENTS, PARTICIPANTS: Men with congenital absence of the vas deferens undergoing sperm aspiration as part of their infertility treatment and control donors who provided ejaculated sperm. RESULTS: Epididymal sperm penetrated SPA with a score of 0% to 30%. The SPA scores for internal controls using ejaculated sperm was 30% to 71%. Linear regression analysis of the association between penetration scores in SPA and fertilization rate in IVF indicated a positive correlation that was highly significative. CONCLUSIONS: These findings using SPA confirm previous reports on the fertilizing potential of human epididymal sperm and its ability to produce normal pregnancies. The good correlation between SPA and human IVF using epididymal sperm suggest that SPA is an excellent bioassay to test laboratory experimental conditions for improving fertilizing capacity of human epididymal sperm.
F J Rojas; A T La; T Ord; P Patrizio; J P Balmaceda; S J Silber; R H Asch
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Fertility and sterility     Volume:  58     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1992-12-04     Completed Date:  1992-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1000-5     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University of California, Irvine Medical Center, Orange 92668.
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MeSH Terms
Epididymis / cytology*
Fertilization in Vitro
Infertility, Male / etiology*,  therapy*
Oocytes / physiology*
Regression Analysis
Sperm-Ovum Interactions*
Spermatozoa / physiology*
Vas Deferens / abnormalities*
Zona Pellucida / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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