Document Detail


Penetration studies of topically applied substances: Optical determination of the amount of stratum corneum removed by tape stripping.
MedLine Citation:
PMID:  17092175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tape stripping is a standard measuring method for the investigation of the dermatopharmacokinetics of topically applied substances using adhesive films. These tape strips are successively applied and removed from the skin after application and penetration of topically applied substances. Thus, layers of corneocytes and some amount of topical applied substances are removed. The amount of substances and the amount of stratum corneum removed with a single tape strip has to be determined for the calculation of the penetration profile. The topically applied substances removed from the skin can be determined by classical analytical methods like high-pressure liquid chromatography, mass spectroscopy, and spectroscopic measurements. The amount of corneocytes on the tape strips can be easily detected by their pseudoabsorption. In the present paper, an easy and cheap corneocyte density analyzer is presented that is based on a slide projector. Comparing the results of the measurements obtained by the corneocyte density analyzer and by uv-visible spectrometry, identical results were obtained.
Authors:
J Lademann; A Ilgevicius; O Zurbau; H D Liess; S Schanzer; H J Weigmann; C Antoniou; R V Pelchrzim; W Sterry
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  11     ISSN:  1083-3668     ISO Abbreviation:  J Biomed Opt     Publication Date:    2006 Sep-Oct
Date Detail:
Created Date:  2006-11-09     Completed Date:  2007-01-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  054026     Citation Subset:  IM    
Affiliation:
Charité-Universitätsmedizin Berlin, Department of Dermatology and Allergology, Center of Experimental and Applied Cutaneous Physiology (CCP), D-10098 Berlin, Germany. juergen.lademann@charite.de
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Adult
Cell Count / instrumentation*,  methods
Clobetasol / administration & dosage*,  pharmacokinetics*
Equipment Design
Equipment Failure Analysis
Humans
Keratinocytes / cytology*,  metabolism*
Optics and Photonics / instrumentation
Photometry / instrumentation,  methods
Skin Absorption / physiology*
Surgical Tape*
Chemical
Reg. No./Substance:
25122-41-2/Clobetasol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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