Document Detail

Peliosis hepatis: microscopic and macroscopic type, time pattern, and correlation with liver cell apoptosis in a model of toxic liver injury.
MedLine Citation:
PMID:  17053957     Owner:  NLM     Status:  MEDLINE    
Macroscopic and microscopic types of peliosis hepatis, time pattern, and correlation with hepatocyte and sinusoidal cell apoptosis were investigated. Male Wistar rats were injected with a dose of cadmium (6.5 mg CdCl(2)/kg body weight, intraperitoneally; group I). Putrescine (300 micromol/kg body weight, intraperitoneally; group II) was injected at 2, 5, and 8 hours and vascular endothelial growth factor (VEGF; 400 ng/animal, intravenously; group III) at 2 hours. Animals from each group were humanely killed 0, 6, 12, 24, 48, or 60 hours after cadmium intoxication. Liver tissue was histologically assessed for necrosis, apoptosis, and peliosis. Apoptosis was also quantified by the TUNEL assay for hepatocytes and nonparenchymal liver cells. The discrimination between hepatic cell subpopulations was done histochemically. Sinusoidal cell apoptosis and macroscopic peliosis hepatis evolved in a monophasic pattern and correlated closely. Putrescine or VEGF administration totally reversed macroscopic peliosis. Putrescine exerted a major protective effect on hepatocytes, whereas the protective effect of VEGF was more pronounced for nonparenchymal liver cells. Microscopic peliosis also evolved in a monophasic pattern preceding macroscopic type. The extent of the lesion was reduced by putrescine and almost totally reversed by VEGF. Macroscopic peliosis progresses as a compound lesion closely correlating with nonparenchymal cell apoptosis. Both hepatocyte and nonparenchymal cell injury are prerequisites for the genesis of the lesion. Microscopic peliosis precedes macroscopic peliosis and up to a degree seems to be independent of initial hepatocyte injury, but it seems to depend on nonparenchymal cell injury.
Konstantinos N Tzirogiannis; George K Papadimas; Vasiliki G Kondyli; Kalliopi T Kourentzi; Maria D Demonakou; Loukas G Kyriakou; Michael G Mykoniatis; Rosa I Hereti; Georgios I Panoutsopoulos
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Publication Detail:
Type:  Journal Article     Date:  2006-10-20
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  51     ISSN:  0163-2116     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-24     Completed Date:  2007-01-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1998-2006     Citation Subset:  AIM; IM    
Department of Experimental Pharmacology, Medical School, Athens University, 5 Tenedou Street, Platia Amerikis, Athens, 112 57, Greece.
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MeSH Terms
Cadmium / adverse effects
Disease Models, Animal
Hepatocytes / physiology*
In Situ Nick-End Labeling
Liver / drug effects,  pathology
Peliosis Hepatis / chemically induced,  pathology*,  physiopathology
Putrescine / pharmacology
Rats, Wistar
Vascular Endothelial Growth Factor A / pharmacology
Reg. No./Substance:
0/Vascular Endothelial Growth Factor A; 110-60-1/Putrescine; 7440-43-9/Cadmium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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