Document Detail

The Pekin duck IL-10R2 common chain: cDNA cloning, genomic structure, molecular characterization and mRNA expression analysis.
MedLine Citation:
PMID:  23331509     Owner:  NLM     Status:  Publisher    
The interleukin-10 receptor 2 (IL-10R2, IL-10Rβ) is required for the signalling of the class 2 cytokines IL-10, IL-22, IL-26 and IFN-λ(1-3) . Here, we describe the identification of the Pekin duck IL-10R2 (duIL-10R2) common chain and its gene structure. The duIL-10R2 cDNA encodes a 343 amino acid protein that has an amino acid identity of 76% and 42% with chicken and human IL-10R2, respectively. Binding residues of human IL-10R2 for IL-10 and IL-22 were mostly conserved in the avian IL-10R2 proteins within loops L3 and L5, but not within loops L2 and L6. Homology modelling of the duIL-10R2 extracellular domain structure using soluble human IL-10R2 (shIL-10R2, PDB ID: 3LQM) as a template revealed a protruding loop L5 and two distinct clefts between loops L2/L3 and L3/L5, similar to shIL-10R2. However, in contrast to the three amino acid β-hairpin loop L2 of shIL-10R2, loop L2 of duIL-10R2 is five residues longer. Residues within a putative Tyk2 binding site were highly conserved across all vertebrate IL-10R2 proteins examined. The duIL-10R2 gene shares a seven exon-six intron structure with chicken and human IL-10R2 genes, but avian genes are more compact. DuIL-10R2 mRNA was constitutively expressed in all tissues. Mitogen stimulation of duck peripheral blood mononuclear cells (PBMC) did not alter transcript levels. Our observations suggest that genomic organization and structural features implicated in multiple cytokine-binding properties of human IL-10R2 are conserved in duck IL-10R2, but the evolutionary changes that appear to have lead to low-affinity cytokine interaction within loop L2 are distinct to mammalian species.
Q Yao; K P Fischer; K Arnesen; D Lorne Tyrrell; K S Gutfreund
Related Documents :
19515839 - Inaccuracy of single-target sequencing for discriminating species of the mycobacterium ...
18194339 - Molecular characterization of the mycoplasma bovis p68 gene, encoding a basic membrane ...
23904739 - Comparison of full-length genomics sequences between dengue virus serotype 3, parental ...
9317049 - Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, flab, from ...
15024109 - Functional and evolutionary genomics of mycobacterium tuberculosis: insights from genom...
24429659 - Pea chloroplast trna(lys) (uuu) gene: transcription and analysis of an intron-containin...
20363249 - A dorsal homolog (fcdorsal) in the chinese shrimp fenneropenaeus chinensis is responsiv...
1962839 - The integration-excision system of the conjugative transposon tn 1545 is structurally a...
7918659 - Nucleotide sequence of the phosphotransacetylase gene of escherichia coli strain k12.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  International journal of immunogenetics     Volume:  -     ISSN:  1744-313X     ISO Abbreviation:  Int. J. Immunogenet.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101232337     Medline TA:  Int J Immunogenet     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 Blackwell Publishing Ltd.
Department of Medicine, University of Alberta, Edmonton, AB, Canada; Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Opioid use for non-cancer pain and risk of myocardial infarction among adults.
Next Document:  Vitamin D accelerates clinical recovery from tuberculosis: results of the SUCCINCT Study [Supplement...