| Pediocin production by recombinant lactic acid bacteria. | |
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MedLine Citation:
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PMID: 12882274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Production of the anti-listerial bacteriocin, pediocin, by lactic acid bacteria (LAB) transformed with the cloning vector pPC418 (Ped+, 9.1 kb) was influenced by composition of media and incubation temperature. Maximum pediocin production, tested against Listeria innocua, by electrotransformants of Lactococcus lactis ssp. lactis was measured in tryptone/lactose/yeast extract medium after 24 h growth at 30 degrees C, while incubation at 40 degrees C was optimum for Ped+ transformants of Streptococcus thermophilus and Enterococcus faecalis. The amount of pediocin produced by S. thermophilus in skim milk and cheese whey supplemented with 0.5% yeast extract was estimated as 51,000 units ml(-1) and 25,000 units ml(-1), respectively. Pediocin production remained essentially unchanged in reconstituted skim milk or whey media diluted up to 10-fold. The results demonstrate the capacity of recombinant strains of LAB to produce pediocin in a variety of growth media including skim milk and inexpensive cheese whey-based media, requiring minimum nutritional supplementation. |
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Authors:
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G A Somkuti; D H Steinberg |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biotechnology letters Volume: 25 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-07-28 Completed Date: 2003-09-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 473-7 Citation Subset: IM |
Affiliation:
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Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. gsomkuti@arserrc.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteriocins / biosynthesis* Cheese Culture Media / metabolism Electroporation / methods Enterococcus faecalis / genetics, metabolism* Gene Expression Regulation, Bacterial Lactococcus lactis / genetics, metabolism* Lactose / metabolism Milk / metabolism Pediococcus / genetics, metabolism* Peptones / metabolism Recombination, Genetic Species Specificity Streptococcus / genetics, metabolism* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Bacteriocins; 0/Culture Media; 0/Peptones; 111745-56-3/pediocin PA-1; 63-42-3/Lactose; 73049-73-7/tryptones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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