Document Detail


Pediocin production by recombinant lactic acid bacteria.
MedLine Citation:
PMID:  12882274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Production of the anti-listerial bacteriocin, pediocin, by lactic acid bacteria (LAB) transformed with the cloning vector pPC418 (Ped+, 9.1 kb) was influenced by composition of media and incubation temperature. Maximum pediocin production, tested against Listeria innocua, by electrotransformants of Lactococcus lactis ssp. lactis was measured in tryptone/lactose/yeast extract medium after 24 h growth at 30 degrees C, while incubation at 40 degrees C was optimum for Ped+ transformants of Streptococcus thermophilus and Enterococcus faecalis. The amount of pediocin produced by S. thermophilus in skim milk and cheese whey supplemented with 0.5% yeast extract was estimated as 51,000 units ml(-1) and 25,000 units ml(-1), respectively. Pediocin production remained essentially unchanged in reconstituted skim milk or whey media diluted up to 10-fold. The results demonstrate the capacity of recombinant strains of LAB to produce pediocin in a variety of growth media including skim milk and inexpensive cheese whey-based media, requiring minimum nutritional supplementation.
Authors:
G A Somkuti; D H Steinberg
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biotechnology letters     Volume:  25     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-07-28     Completed Date:  2003-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  473-7     Citation Subset:  IM    
Affiliation:
Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA. gsomkuti@arserrc.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriocins / biosynthesis*
Cheese
Culture Media / metabolism
Electroporation / methods
Enterococcus faecalis / genetics,  metabolism*
Gene Expression Regulation, Bacterial
Lactococcus lactis / genetics,  metabolism*
Lactose / metabolism
Milk / metabolism
Pediococcus / genetics,  metabolism*
Peptones / metabolism
Recombination, Genetic
Species Specificity
Streptococcus / genetics,  metabolism*
Temperature
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/Culture Media; 0/Peptones; 111745-56-3/pediocin PA-1; 63-42-3/Lactose; 73049-73-7/tryptones

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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