| Pectin-supplemented enteral diet reduces the severity of methotrexate induced enterocolitis in rats. | |
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MedLine Citation:
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PMID: 8789894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Administration of methotrexate (MTX) to rats fed an elemental diet results in a high mortality from severe enterocolitis. Previous studies have shown that pectin is an important precursor of substrates for intestinal structure and function and may facilitate intestinal recovery after enterocolitis. The aim of this study is to evaluate the effect of pectin on MTX-induced enterocolitis in rats. METHODS: Rats received intragastric infusion of either 1% pectin-supplemented or pectin-free elemental diet from the beginning of the study via a gastrostomy. On the 4th day animals received either MTX, 20 mg/kg intraperitoneally, or saline injection and were killed on the 7th day for sampling. RESULTS: Pectin supplementation significantly decreased body weight loss, organ water content, and intestinal myeloperoxidase levels and increased mucosal protein, DNA, and RNA content in enterocolitis rats. The intestinal permeability was increased by administration of MTX, and pectin supplementation significantly reversed the increased permeability in the distal small bowel and colon. Pectin supplementation also lowered the magnitude of bacterial translocation, decreased plasma endotoxin levels, and restored bowel microecology. CONCLUSIONS: Pectin significantly decreased MTX-induced intestinal injury and improved bowel integrity. |
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Authors:
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Y Mao; B Kasravi; S Nobaek; L Q Wang; D Adawi; G Roos; U Stenram; G Molin; S Bengmark; B Jeppsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of gastroenterology Volume: 31 ISSN: 0036-5521 ISO Abbreviation: Scand. J. Gastroenterol. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-11-13 Completed Date: 1996-11-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060105 Medline TA: Scand J Gastroenterol Country: NORWAY |
Other Details:
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Languages: eng Pagination: 558-67 Citation Subset: IM |
Affiliation:
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Dept. of Surgery, Lund University, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Translocation Cecum / microbiology Dietary Fiber / administration & dosage, therapeutic use* Endotoxemia / prevention & control Enteral Nutrition* Enterocolitis / chemically induced, diet therapy* Food, Formulated* Ileum / microbiology Intestinal Absorption Intestinal Mucosa / metabolism, pathology Male Methotrexate* Pectins / administration & dosage, therapeutic use* Peroxidase / metabolism Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Pectins; 59-05-2/Methotrexate; 9000-69-5/pectin; EC 1.11.1.7/Peroxidase |
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