Document Detail


Pectin may hinder the unfolding of xyloglucan chains during cell deformation: implications of the mechanical performance of Arabidopsis hypocotyls with pectin alterations.
MedLine Citation:
PMID:  19825674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plant cell walls, like a multitude of other biological materials, are natural fiber-reinforced composite materials. Their mechanical properties are highly dependent on the interplay of the stiff fibrous phase and the soft matrix phase and on the matrix deformation itself. Using specific Arabidopsis thaliana mutants, we studied the mechanical role of the matrix assembly in primary cell walls of hypocotyls with altered xyloglucan and pectin composition. Standard microtensile tests and cyclic loading protocols were performed on mur1 hypocotyls with affected RGII borate diester cross-links and a hindered xyloglucan fucosylation as well as qua2 exhibiting 50% less homogalacturonan in comparison to wild-type. As a control, wild-type plants (Col-0) and mur2 exhibiting a specific xyloglucan fucosylation and no differences in the pectin network were utilized. In the standard tensile tests, the ultimate stress levels (approximately tensile strength) of the hypocotyls of the mutants with pectin alterations (mur1, qua2) were rather unaffected, whereas their tensile stiffness was noticeably reduced in comparison to Col-0. The cyclic loading tests indicated a stiffening of all hypocotyls after the first cycle and a plastic deformation during the first straining, the degree of which, however, was much higher for mur1 and qua2 hypocotyls. Based on the mechanical data and current cell wall models, it is assumed that folded xyloglucan chains between cellulose fibrils may tend to unfold during straining of the hypocotyls. This response is probably hindered by geometrical constraints due to pectin rigidity.
Authors:
Willie Abasolo; Michaela Eder; Kazuchika Yamauchi; Nicolai Obel; Antje Reinecke; Lutz Neumetzler; John W C Dunlop; Gregory Mouille; Markus Pauly; Herman H??fte; Ingo Burgert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-04
Journal Detail:
Title:  Molecular plant     Volume:  2     ISSN:  1674-2052     ISO Abbreviation:  Mol Plant     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-10-14     Completed Date:  2010-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465514     Medline TA:  Mol Plant     Country:  England    
Other Details:
Languages:  eng     Pagination:  990-9     Citation Subset:  IM    
Affiliation:
Max-Planck-Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  metabolism*
Cell Wall / metabolism,  physiology
Cellulose / metabolism
Glucans / metabolism*
Hypocotyl / genetics,  metabolism*
Models, Theoretical
Pectins / genetics,  metabolism*
Plants, Genetically Modified / genetics,  metabolism
Tensile Strength / physiology
Xylans / metabolism*
Chemical
Reg. No./Substance:
0/Glucans; 0/Pectins; 0/Xylans; 37294-28-3/xyloglucan; 9004-34-6/Cellulose; 9046-38-2/polygalacturonic acid

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