Document Detail

Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells.
MedLine Citation:
PMID:  18809620     Owner:  NLM     Status:  MEDLINE    
The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum has previously been reported to consist of arabinogalactans types I and II (AG-I and AG-II), probably linked to a rhamnogalacturonan backbone. We describe here further structural characteristics of the main polysaccharide fraction (BP1002) and fractions obtained by enzymatic degradations using endo-alpha-d-(1-->4)-polygalacturonase (BP1002-I to IV). The results indicate that in addition to previously reported structures, rhamnogalacturan type II and xylogalacturonan areas appear to be present in the pectic polymer isolated from the plant. Atomic force microscopy confirmed the presence of branched structures, as well as a polydisperse nature. We further tested whether the BP1002 main fraction or the enzymatically degraded products could induce immunomodulating activity through stimulation of subsets of leukocytes. We found that macrophages and dendritic cells were activated by BP1002 fractions, while there was little response of T cells, B cells, and NK cells. The enzymatic treatment of the BP1002 main fraction gave important information on the structure-activity relations. It seems that the presence of rhamnogalacturonan type I is important for the bioactivity, as the bioactivity decreases with the decreased amounts of rhamnose, galactose, and arabinose. The demonstration of bioactivity by the plant extracts might indicate the mechanisms behind the traditional medical use of the plant.
Marit Inngjerdingen; Kari T Inngjerdingen; Trushar R Patel; Stephanie Allen; Xinyong Chen; Bent Rolstad; Gordon A Morris; Stephen E Harding; Terje E Michaelsen; Drissa Diallo; Berit S Paulsen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-22
Journal Detail:
Title:  Glycobiology     Volume:  18     ISSN:  1460-2423     ISO Abbreviation:  Glycobiology     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-27     Completed Date:  2009-02-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9104124     Medline TA:  Glycobiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  1074-84     Citation Subset:  IM    
Department of Pharmaceutical Chemistry, School of Pharmacy, P.O. Box 1068, Blindern, N-0316 Oslo, Norway.
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MeSH Terms
Angiosperms / chemistry*
Antigen-Presenting Cells / metabolism
Dendritic Cells / drug effects*,  metabolism
Leukocytes / metabolism
Lymphocytes / metabolism
Macrophages / drug effects*,  metabolism
Microscopy, Atomic Force
Models, Molecular
Molecular Weight
Pectins / chemistry*,  isolation & purification,  pharmacology*
Plant Extracts / chemistry,  metabolism
Polygalacturonase / metabolism
Rats, Inbred Strains
Structure-Activity Relationship
Reg. No./Substance:
0/Pectins; 0/Plant Extracts; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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