Document Detail

Pectic polysaccharide rhamnogalacturonan II is covalently linked to homogalacturonan.
MedLine Citation:
PMID:  11423143     Owner:  NLM     Status:  MEDLINE    
A borate-containing pectin was solubilized from sugar beet (Beta vulgaris L. ) cell walls by treatment with 0.5 M imidazole, pH 7. The molecular weight of the pectin was reduced when the borate ester was hydrolyzed by treatment with 1 N HCl. Treatment of the acid-treated pectin with boric acid in the presence of Pb(2+) gave a product whose molecular weight distribution was similar to the imidazole-soluble pectin. The imidazole-soluble pectin was saponified and then digested with endo- and exo-polygalacturonases. These treatments shifted the boron peak at the high molecular weight region to the low molecular weight (10 kDa), which corresponds to rhamnogalacturonan II-borate ester cross-linked dimer (dRG-II-B). The treatment also generated rhamnogalacturonan I (RG-I), dRG-II-B, monomeric rhamnogalacturonan II and galacturonic acid. These results show that imidazole solubilizes a high molecular weight borate-containing pectic complex composed of homogalacturonan-rhamnogalacturonan II and RG-I. Our data suggest that borate esters formed between rhamnogalacturonan II molecules cross-link the macromolecular pectin.
T Ishii; T Matsunaga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  57     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-06-25     Completed Date:  2001-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  969-74     Citation Subset:  IM    
Forestry and Forest Products Research Institute, PO Box 16, Tsukuba Norin Kenkyu, Danchi-nai, 305-8687, Ibaraki, Japan.
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MeSH Terms
Cell Wall / chemistry
Chenopodiaceae / chemistry*
Chromatography, Gel
Chromatography, High Pressure Liquid
Molecular Weight
Monosaccharides / analysis
Pectins / chemistry*,  isolation & purification
Reg. No./Substance:
0/Borates; 0/Monosaccharides; 0/Pectins; 0/rhamnogalacturonan II; 9046-38-2/polygalacturonic acid; EC

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