Document Detail


Pearls are novel Cajal body-like structures in the Xenopus germinal vesicle that are dependent on RNA pol III transcription.
MedLine Citation:
PMID:  23135638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified novel nuclear bodies, which we call pearls, in the giant oocyte nuclei of Xenopus laevis and Xenopus tropicalis. Pearls are attached to the lampbrush chromosomes at specific loci that are transcribed by RNA polymerase III, and they disappear after inhibition of polymerase III activity. Pearls are enriched for small Cajal body-specific RNAs (scaRNAs), which are guide RNAs that modify specific nucleotides on splicing snRNAs. Surprisingly, snRNAs themselves are not present in pearls, suggesting that pearls are not functionally equivalent to Cajal bodies in other systems, which contain both snRNAs and scaRNAs. We suggest that pearls may function in the processing of RNA polymerase III transcripts, such as tRNA, 5S rRNA, and other short non-coding RNAs.
Authors:
Zehra F Nizami; Joseph G Gall
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  20     ISSN:  1573-6849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-02-06     Completed Date:  2013-07-26     Revised Date:  2014-05-21    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  953-69     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Chromosomes / genetics,  ultrastructure
Cloning, Molecular
Coiled Bodies / genetics*,  ultrastructure
Genetic Loci
In Situ Hybridization, Fluorescence
Oocytes / cytology,  metabolism
RNA / analysis*,  genetics,  metabolism
RNA Polymerase III / genetics*,  metabolism
RNA Splicing
RNA, Ribosomal, 5S / genetics,  metabolism
RNA, Small Nucleolar / genetics,  metabolism
Transcription, Genetic
Xenopus laevis / genetics*
Grant Support
ID/Acronym/Agency:
P40 OD010997/OD/NIH HHS; R01 GM033397/GM/NIGMS NIH HHS; R01 GM33397/GM/NIGMS NIH HHS; T32 GM007231/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Ribosomal, 5S; 0/RNA, Small Nucleolar; 0/RNA, U3 small nucleolar; 63231-63-0/RNA; EC 2.7.7.-/RNA Polymerase III
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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