Document Detail


Peanut protein allergens: gastric digestion is carried out exclusively by pepsin.
MedLine Citation:
PMID:  15356566     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A major characteristic of many food allergens, including Ara h 1, a major peanut allergen, is their resistance to gastric digestion. One estimate of the allergenic potential of a possible protein allergen is its stability under simulated gastric conditions. OBJECTIVE: Because the rate and extent of digestion of allergenic proteins will affect the severity of any subsequent allergic response, it is important to correlate protein allergen digestion in simulated gastric fluid with that in actual gastric fluid. METHODS: A major peanut allergen, Ara h 1, was digested in vitro by using both pepsin and porcine gastric fluid. Several comparisons between the 2 sets of proteolytic conditions were assessed including pH optima and the effect of temperature, denaturants, and specific enzyme inhibitors. RESULTS: In vitro digestion of Ara h 1 with pepsin and porcine gastric fluid resulted in virtually identical hydrolysis patterns as observed on SDS-PAGE. The protease activity of both pepsin and gastric fluid were inhibited at high pH and in the presence of pepstatin. However, both remained active in 4 mol/L urea and at 60 degrees C. CONCLUSIONS: Protein digestion in the porcine stomach is carried out by pepsin. In vivo gastric digestion is modeled accurately by peptic hydrolysis. Digestion conditions in vivo are comparable to experimental conditions in vitro provided that the acidic nature of the stomach contents is optimal for characterization of the allergen under standard pepsin digestion conditions. Additional experimentation using crude food extracts, both in the presence and absence of a complete meal, is needed to elucidate the complete physiologic nature of food allergen digestion.
Authors:
Randall A Kopper; N Joey Odum; Moon Sen; Ricki M Helm; J Steve Stanley; A Wesley Burks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  114     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-09     Completed Date:  2004-10-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  614-8     Citation Subset:  AIM; IM    
Affiliation:
Chemistry Department, Hendrix College, Conway, Arkansas, USA.
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MeSH Terms
Descriptor/Qualifier:
Allergens / adverse effects,  chemistry,  metabolism
Animals
Arachis hypogaea / adverse effects*,  chemistry,  immunology
Digestion*
Endopeptidases / metabolism
Gastric Juice / enzymology
Hydrogen-Ion Concentration
Nut Hypersensitivity*
Pepsin A / metabolism*
Plant Proteins / adverse effects,  chemistry,  metabolism
Swine
Chemical
Reg. No./Substance:
0/Allergens; 0/Plant Proteins; EC 3.4.-/Endopeptidases; EC 3.4.23.1/Pepsin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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