Document Detail


Peamaclein--a new peach allergenic protein: similarities, differences and misleading features compared to Pru p 3.
MedLine Citation:
PMID:  23278887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Among the peach-derived allergens which are already known, the lipid transfer protein (Pru p 3) seems to be the one to exert severe allergic reactions.
OBJECTIVE: To identify and characterize a new peach allergen causing a clinical picture similar to that of Pru p 3.
METHODS: Patients were selected on the basis of their severe clinical reactivity and negative results to a panel of peach allergens available on the ISAC103 microarray. Several in-house and commercial preparations were compared. Several methods were used to characterize the newly identified molecule. Specific IgE and inhibition assays were performed using the Allergen micro-Beads Array (ABA) assay.
RESULTS: Negative ISAC results to Pru p 3 were confirmed by additional testing in contrast with the positive results obtained by commercial Pru p 3-enriched peach peel extracts. The analyses of one of these preparations led to the identification of Peamaclein, a new allergenic protein. It is a small, basic, cysteine-rich, heat-stable, digestion-resistant protein, homologous to a potato antimicrobial peptide. Peamaclein was able to trigger positive skin test reactions and to bind IgE in the ABA assay. It displays an electrophoretic mobility and chromatographic behaviour similar to that of Pru p 3; therefore, it can be hidden in Pru p 3 preparations. In fact, Pru p 3-enriched peach peel extracts were found to contain both Pru p 3 and Peamaclein by means of comparative in vivo testing, and by biochemical and immunochemical assays. Commercially available anti-Pru p 3 polyclonal antibodies were found to have a double specificity for the two molecules.
CONCLUSIONS AND CLINICAL RELEVANCE: A new allergen from peach belonging to a new family of allergenic proteins has been identified and characterized. This knowledge on Peamaclein will improve our understanding on the clinical aspects of the peach allergy and the quality of diagnostic reagents.
Authors:
L Tuppo; C Alessandri; D Pomponi; D Picone; M Tamburrini; R Ferrara; M Petriccione; I Mangone; P Palazzo; M Liso; I Giangrieco; R Crescenzo; M L Bernardi; D Zennaro; M Helmer-Citterich; A Mari; M A Ciardiello
Related Documents :
22484057 - A proteomics strategy for determining the synthesis and degradation rates of individual...
16081577 - Intracellular antibodies (intrabodies) versus rna interference for therapeutic applicat...
10189717 - Proteomics: quantitative and physical mapping of cellular proteins.
21048197 - Highly reproducible label free quantitative proteomic analysis of rna polymerase comple...
18619997 - Intrinsic disorder in scaffold proteins: getting more from less.
9453057 - Analysis of drug/plasma protein interactions by means of asymmetrical flow field-flow f...
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  43     ISSN:  1365-2222     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-07-25     Revised Date:  2014-08-16    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  128-40     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Allergens / adverse effects,  chemistry,  immunology*
Antigens, Plant / adverse effects,  chemistry,  immunology*
Child
Child, Preschool
Double-Blind Method
Female
Food Hypersensitivity / immunology*
Humans
Immunoglobulin E / biosynthesis
Male
Middle Aged
Plant Extracts / chemistry,  immunology
Plant Proteins / adverse effects,  chemistry,  immunology*
Prunus / adverse effects,  chemistry,  immunology*
Young Adult
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Plant; 0/Plant Extracts; 0/Plant Proteins; 0/Pru p 3 allergen; 0/peamaclein protein, Prunus persica; 37341-29-0/Immunoglobulin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  In silico prediction of Ara h 2 T cell epitopes in peanut-allergic children.
Next Document:  Patterns of cranial ontogeny in lacertid lizards: morphological and allometric disparity.