Document Detail


Pdc1 functions in the assembly of P bodies in Schizosaccharomyces pombe.
MedLine Citation:
PMID:  23319050     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
P bodies are cytoplasmic RNA granules containing the Dcp1-Dcp2 decapping enzymes where mRNA decay can occur. Here, we describe the characterization of P bodies in the fission yeast Schizosaccharomyces pombe. Most information on the property and function of P bodies stems from studies in the distantly related budding yeast Saccharomyces cerevisiae, and Edc3 was identified as a scaffold protein required for P-body assembly. However, we found that, unlike in S. cerevisiae, fission yeast Edc3 was dispensable for P-body formation. Pdc1, a novel partner of the fission yeast decapping enzyme, with a limited similarity to plant Edc4/Varicose that is required for the assembly of P bodies, was identified (tandem affinity purification-matrix-assisted laser desorption ionization tandem mass spectrometry [TAP-MALDI MS/MS]). Pdc1 interacts with Dcp2 through its C terminus and contains a coiled-coil region for self-interaction to mediate P-body formation. In line with the model that Pdc1 cross-bridges different proteins, additional interactions can be demonstrated with components such as Edc3 and Ste13. Although Pdc1 is not required for the interaction between Dcp1 and Dcp2, our data suggest that Pdc1 acts as a functional homologue of Edc4, a third component of the decapping enzymes that is thought to be absent from fungi. Together, these results highlight the diverse P-body protein compositions between different species and might help to provide insight into their evolutionary paths.
Authors:
Chun-Yu Wang; Wen-Ling Chen; Shao-Win Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-14
Journal Detail:
Title:  Molecular and cellular biology     Volume:  33     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-22     Completed Date:  2013-07-05     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1244-53     Citation Subset:  IM    
Affiliation:
Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan Town, Miaoli County, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cytoplasmic Granules / genetics*,  metabolism*
HeLa Cells
Humans
RNA, Fungal / genetics
Schizosaccharomyces / genetics*,  metabolism*
Schizosaccharomyces pombe Proteins / genetics*,  metabolism*
Chemical
Reg. No./Substance:
0/Dcp1 protein, S pombe; 0/Dcp2 protein, S pombe; 0/RNA, Fungal; 0/Schizosaccharomyces pombe Proteins; 0/enhancer of decapping 3 protein, S pombe
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