Document Detail


The PbMDJ1 gene belongs to a conserved MDJ1/LON locus in thermodimorphic pathogenic fungi and encodes a heat shock protein that localizes to both the mitochondria and cell wall of Paracoccidioides brasiliensis.
MedLine Citation:
PMID:  16467478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
J-domain (DnaJ) proteins, of the Hsp40 family, are essential cofactors of their cognate Hsp70 chaperones, besides acting as independent chaperones. In the present study, we have demonstrated the presence of Mdj1, a mitochondrial DnaJ member, not only in the mitochondria, where it is apparently sorted, but also in the cell wall of Paracoccidioides brasiliensis, a thermodimorphic pathogenic fungus. The molecule (PbMdj1) was localized to fungal yeast cells using both confocal and electron microscopy and also flow cytometry. The anti-recombinant PbMdj1 antibodies used in the reactions specifically recognized a single 55-kDa mitochondrial and cell wall (alkaline beta-mercaptoethanol extract) component, compatible with the predicted size of the protein devoid of its matrix peptide-targeting signal. Labeling was abundant throughout the cell wall and especially in the budding regions; however, anti-PbMdj1 did not affect fungal growth in the concentrations tested in vitro, possibly due to the poor access of the antibodies to their target in growing cells. Labeled mitochondria stood preferentially close to the plasma membrane, and gold particles were detected in the thin space between them, toward the cell surface. We show that Mdj1 and the mitochondrial proteinase Lon homologues are heat shock proteins in P. brasiliensis and that their gene organizations are conserved among thermodimorphic fungi and Aspergillus, where the genes are adjacent and have a common 5' region. This is the first time a DnaJ member has been observed on the cell surface, where its function is speculative.
Authors:
Wagner L Batista; Alisson L Matsuo; Luciane Ganiko; Tânia F Barros; Thiago R Veiga; Edna Freymüller; Rosana Puccia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Eukaryotic cell     Volume:  5     ISSN:  1535-9778     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-09     Completed Date:  2006-05-08     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  379-90     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Immunology and Parasitilogy, Federal University of São Paulo, SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / metabolism*,  ultrastructure
Conserved Sequence*
Flow Cytometry
Genome, Fungal
HSP40 Heat-Shock Proteins / chemistry,  genetics*,  metabolism*
Humans
Introns / genetics
Mitochondria / metabolism*,  ultrastructure
Paracoccidioides / cytology*,  genetics*,  metabolism,  pathogenicity
Paracoccidioidomycosis
Phylogeny
Protein Transport
Recombinant Fusion Proteins
Chemical
Reg. No./Substance:
0/HSP40 Heat-Shock Proteins; 0/Recombinant Fusion Proteins
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