Document Detail


Pax9 mediated cell survival in oral squamous carcinoma cell enhanced by c-myb.
MedLine Citation:
PMID:  18979497     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Paired box gene 9 (Pax9) and c-myb are transcription factors that regulate the expression of the genes involved in mediating cell proliferation, resistance to apoptosis, and migration. However, the function of Pax9 in oral squamous cell carcinoma (OSCC) is virtually unknown. This study examined the anti-apoptotic roles of Pax9 and c-myb, and clarified interaction between the two genes in KB cells. Inhibition of Pax9 caused the induction of apoptosis with enhanced cleavage of caspase-3 and PARP, accelerated Bax, and reduced Bcl-2 expression. Transducing c-myb cells with adenovirus c-myb (Ad/c-myb) were induced cell growth and inhibited apoptosis, but dominant-negative myb cells (Ad/DN-myb) were not affected. Pax9 was upregulated in the Ad/c-myb cells with simultaneous decrease in the Ad/DN-myb infection. However, c-myb remained unaffected in the Pax9 small interfering RNA (siRNA) transfected cells. Moreover, the Pax9 siRNA transfected cells and Ad/DN-myb infected cells were able to arrest the cell cycle at the G(0) phase. This suggests that Pax9 and c-myb expression in KB cells is essential for cell growth, and survival is enhanced by c-myb. Disrupting the function of c-myb and Pax9 could be a potential target for cancer treatment.
Authors:
Jung-Chang Lee; Manju Sharma; Young-Hee Lee; Nan-Hee Lee; Sun-Young Kim; Ji-Soo Yun; Sang-Yun Nam; Pyoung-Han Hwang; Eun-Chung Jhee; Ho-Keun Yi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  26     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-03     Completed Date:  2009-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  892-9     Citation Subset:  IM    
Affiliation:
Department of Oral Biochemistry, Institute of Oral-Bio Science, School of Dentistry, Chonbuk National University, Joenju, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics,  metabolism
Apoptosis
Carcinoma, Squamous Cell / genetics*,  metabolism
Cell Cycle
Cell Line, Tumor
Cell Survival
G0 Phase
Humans
KB Cells
Mouth Neoplasms / genetics*,  metabolism
PAX9 Transcription Factor / genetics*,  metabolism
Proto-Oncogene Proteins c-myb / genetics*,  metabolism
RNA, Messenger / metabolism
RNA, Small Interfering / genetics
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/PAX9 Transcription Factor; 0/PAX9 protein, human; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Messenger; 0/RNA, Small Interfering

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