Document Detail


Pax6-induced alteration of cell fate: shape changes, expression of neuronal alpha tubulin, postmitotic phenotype, and cell migration.
MedLine Citation:
PMID:  16425216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transcription factor Pax6 plays an important role in the development of the central nervous system. To understand its mechanism of action, we transduced HeLa cells with a Pax6-expressing lentiviral vector. Upon transduction, HeLa cells markedly changed shape and formed neuritelike extensions. Pax6-transduced HeLa cells expressed high levels of neuronal alpha3 tubulin, demonstrating a partial transdifferentiation towards a neuronal phenotype. Neurons are postmitotic cells. Pax6-transduced HeLa cells became postmitotic through mechanisms involving up-regulation of p53 and cyclin-dependent kinase inhibitor p21. One of the most striking effects of Pax6 was observed by time-lapse videomicroscopy: cells started to dissociate from cell clusters and displayed intense migratory activity. Migration was accompanied by dynamic and reversible shape changes. Our results identified three elements of Pax6 action: (i) expression of neuron-specific genes; (ii) establishment of a postmitotic phenotype; and (iii) involvement in the regulation of cell shape and cell migration.
Authors:
Laetitia Cartier; Terese Laforge; Anis Feki; Serge Arnaudeau; Michel Dubois-Dauphin; Karl-Heinz Krause
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurobiology     Volume:  66     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-23     Completed Date:  2006-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  421-36     Citation Subset:  IM    
Affiliation:
Department of Geriatrics, Biology of Ageing Laboratory, Geneva University Hospitals, 1225 Chêne-Bourg, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology
Cell Differentiation / physiology*
Cell Division / physiology
Cell Lineage / physiology
Cell Movement / physiology*
Cell Shape / physiology
Cells, Cultured
Central Nervous System / cytology,  embryology*,  metabolism
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
Eye Proteins / genetics,  metabolism*
Hela Cells
Homeodomain Proteins / genetics,  metabolism*
Humans
Mice
Neurites / metabolism,  ultrastructure
Neurons / cytology,  metabolism*
Paired Box Transcription Factors / genetics,  metabolism*
Phenotype
Repressor Proteins / genetics,  metabolism*
Transduction, Genetic
Tubulin / metabolism*
Tumor Suppressor Protein p53 / metabolism
Chemical
Reg. No./Substance:
0/Cyclin-Dependent Kinase Inhibitor p21; 0/Eye Proteins; 0/Homeodomain Proteins; 0/PAX6 protein; 0/Paired Box Transcription Factors; 0/Repressor Proteins; 0/Tubulin; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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