Document Detail


Pax3( GFP ) ,a new reporter for the melanocyte lineage, highlights novel aspects of PAX3 expression in the skin.
MedLine Citation:
PMID:  22621661     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The paired box gene 3 (Pax3) is expressed during pigment cell development. We tested whether the targeted allele Pax3(GFP) can be used as a reporter gene for pigment cells in the mouse. We found that the enhanced Green Fluorescent Protein (GFP) can readily be seenin every melanoblastand melanocyte in the epidermis and hair follicles of Pax3(GFP/+) heterozygotes. The GFP was detected at all differentiation stages, including melanocyte stem cells. In the dermis, Schwann cells and nestin-positive cells of the piloneural collars resembling the nestin-positive hair follicle multipotent stem cells exhibited a weaker GFP signal. Pigment cells could be purified by fluorescent activated cell sorting and grown in vitrowithout feeder cells, giving pure cultures of melanocytes.The Schwann cells and nestin-positive cells of the piloneural collars were FACS-isolated basedon their weak expression of GFP. Thus Pax3(GFP) can discriminate distinct populations of cells in the skin. Significance:  In this report, the Pax3(GFP) allele is validated as a convenient and sensitive reporter for pigment cells in the epidermis and hair follicles. Pax3(GFP) provides a FACS usable marker formelanoblasts and melanocytes from embryonic and neonatal skin and can be used to obtain pure cultures of melanocytes. © 2012 John Wiley & Sons A/S.
Authors:
Johanna Djian-Zaouche; Cécile Campagne; Edouard Reyes-Gomez; Stéphanie Gadin-Czerw; Florence Bernex; Anne Louise; Frédéric Relaix; Margaret Buckingham; Jean-Jacques Panthier; Geneviève Aubin-Houzelstein
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-23
Journal Detail:
Title:  Pigment cell & melanoma research     Volume:  -     ISSN:  1755-148X     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318927     Medline TA:  Pigment Cell Melanoma Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
INRA, UMR955 Génétique Fonctionnelle et Médicale, Ecole Nationale Vétérinaire d'Alfort, 7 avenue du Général de Gaulle, Maisons-Alfort, F-94704 France Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, UMR955 Génétique Fonctionnelle et Médicale7 avenue du Général de Gaulle, Maisons-Alfort, F-94704 France CNRS URA 2578, Département de Biologie du Développement; USC INRA 2026,Pasteur Institute,Unité de Génétique Fonctionnelle de la Souris, 25 rue du Docteur Roux, Paris, F-75724 France Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, Unité d'Embryologie, d'Histologie et d'Anatomie Pathologique, 7 avenue du Général de Gaulle, Maisons-Alfort, F-94704 France Plate-forme de Cytométrie, Département d'Immunologie, Institut Pasteur, Paris, F-75724 France CNRS URA 2578, Département de Biologie du Développement; Institut Pasteur, Unité de Génétique Moléculaire du Développement, 25 rue du Docteur Roux, Paris, F-75724 France RHEM-Réseau d'histologie Expérimentale de Montpellier, Institut de Recherche en Cancérologie de Montpellier, INSERM U896, Centre de Luttecontre Le Cancer Paul Lamarque Val d'Aurelle, Montpellier, F 34298,France.
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