Document Detail

Patterns of fibronectin gene expression and splicing during cell migration in chicken embryos.
MedLine Citation:
PMID:  3256468     Owner:  NLM     Status:  MEDLINE    
A variety of evidence suggests that fibronectin (FN) promotes cell migration during embryogenesis, and it has been suggested that the deposition of FN along migratory pathways may also play a role in cell guidance. In order to investigate such a role for FN, it is important to determine the relative contribution of migrating and pathway-forming cells to the FN in the migratory track, as any synthesis of FN by the migrating cells might be expected to mask guidance cues provided by the exogenous FN from pathway-forming cells. We have therefore used in situ hybridization to determine in developing chicken embryos the distribution and alternative splicing of FN mRNA during three different cell migrations known to occur through FN-rich environments; neural crest cell migration, mesenchymal cell migration in the area vasculosa and endocardial cushion cell migration in the heart. Our results show that trunk neural crest cells do not contain significant FN mRNA during their initial migration. In contrast, migrating mesenchymal cells of the area vasculosa and endocardial cushion cells both contain abundant FN mRNA. Furthermore, the FN mRNA in these migrating mesenchymal and endocardial cells appears to be spliced in a manner identical with that present in the cells adjacent to their pathways. This in vivo evidence for FN synthesis by migrating and pathway cells argues against a generalized role for exogenously produced FN as a guidance mechanism for cell migration.
C Ffrench-Constant; R O Hynes
Related Documents :
8297038 - Mammalian neural crest and neural crest derivatives.
2806118 - A whole-mount immunocytochemical analysis of the expression of the intermediate filamen...
10757508 - Increased expression of p75ntr by neural crest-derived cells in vivo during mitosis.
10899008 - A region of the vertebrate neural plate in which neighbouring cells can adopt neural or...
1351288 - Cytoprotective effect of reduced glutathione in hydrogen peroxide-induced endothelial c...
16500648 - Pathogenicity of salmonella: sope-mediated membrane ruffling is independent of inositol...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  104     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-10-26     Completed Date:  1989-10-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  369-82     Citation Subset:  IM    
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Movement
Chick Embryo
DNA, Recombinant*
Fetal Heart / physiology,  ultrastructure
Fibronectins / biosynthesis,  genetics*
Mesoderm / physiology,  ultrastructure
Neural Crest / physiology
Nucleic Acid Hybridization
RNA Probes
Grant Support
Reg. No./Substance:
0/DNA, Recombinant; 0/Fibronectins; 0/RNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Initial limb budding is independent of apical ectodermal ridge activity; evidence from a limbless mu...
Next Document:  Beige granules as a cell marker for clonal analysis in kidney and liver of mouse aggregation chimaer...