Document Detail

Patterns of factor-VIII related antigen on crossed immunoelectrophoresis and large pore polyacrylamide gel-crossed immunoelectrophoresis in von Willebrand's disease.
MedLine Citation:
PMID:  6775679     Owner:  NLM     Status:  MEDLINE    
Plasma samples from patients with various types of von Willebrand's disease were subdivided into six patterns according to the electrophoretic mobility and shape of VIIIR: Ag on crossed immunoelectrophoresis (CIE): pattern 1 no precipitation are, pattern 2 normal mobility with low arc, arc, pattern 3 intermediate mobility with low arc, pattern 4 faster anodal mobility with low arc, pattern 5 normal mobility with normal are height, pattern 6 faster anodal mobility with normal are height. Of 62 patients, 14 had pattern 1, 6 pattern 2, 16 pattern 3, 12 pattern 4, 9 pattern 5, and 5 pattern 6. Large pore polyacrylamide-agarose gel-crossed immunoelectrophoresis (PAAG-CIE) of crude factor VIII fraction from cryoprecipitate revealed no arcs in patients with pattern 1, three arcs of reduced height in the patients with patterns 2 and 3, four arcs very similar to normal control in patients with pattern 5, and two arcs with fast anodal migration in the patients with patterns 4 and 6. Crude factor VIII fractions from normal cryosupernatant showed one low fast anodally migrating arc corresponding to the fourth arc of normal cryoprecipitate. No peak was seen in patients with pattern 1, and one low fast anodal arc similar to normal control was present in the patients with patterns 2, 3, 4, 5 and 6.
H Fukui; S Mikami; T Takase; Y Fujimura; M Nishino; A Yoshioka
Related Documents :
21397339 - Disease-associated inflammatory biomarker profiles in blood in different subtypes of mu...
1829489 - Systemic effects of collagen-impregnated aortoiliac dacron vascular prostheses on plate...
1710009 - Effect of intraoperative aprotinin administration on postoperative bleeding in patients...
1412199 - Plasminogen activators and plasminogen activator inhibitor 1 before and after venous oc...
6686949 - Chronic immune thrombocytopenic purpura in hemophilia a.
21704889 - Efficacy of sublingual immunotherapy with house dust mite extract in polyallergen sensi...
22448309 - Should we look for celiac disease among all patients with liver function test abnormali...
18640019 - Anca-associated lung fibrosis: analysis of 17 patients.
10776989 - Turner's syndrome and thyroid disease: a transverse study of pediatric patients in brazil.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of haematology     Volume:  46     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1980 Oct 
Date Detail:
Created Date:  1981-01-16     Completed Date:  1981-01-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  269-76     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens / analysis*
Electrophoresis, Polyacrylamide Gel
Factor VIII / immunology*
Immunoelectrophoresis, Two-Dimensional
von Willebrand Diseases / blood,  immunology*
Reg. No./Substance:
0/Antigens; 9001-27-8/Factor VIII

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Decreased association of 45calcium with platelets unable to aggregate due to thrombasthenia or prolo...
Next Document:  Immunochemical analysis of active and inactive antithrombin III.