Document Detail

Patterns of cell death induced by eluates from denture base acrylic resins in U-937 human monoblastoid cells.
MedLine Citation:
PMID:  10706479     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to investigate in vitro the apoptosis- and necrosis-inducing potential of eluates from three heat-polymerized and four autopolymerized poly(methyl methacrylate)-based denture base resins. Our hypothesis was that the rate of cell death by apoptosis and/or necrosis induced by such denture base resins could be an important indicator of their cytotoxicity degree. U-937 human monoblastoid cells were exposed for 24 h and 48 h to eluates of 0.1 g/ml, 0.2 g/ml, 0.4 g/ml, and 0.8 g/ml extracted for 24 h and 48 h. The characteristics of apoptosis and necrosis were evaluated by flow cytometry and light and electron microscopy. Eluates from all resins enhanced cell death by apoptosis and necrosis in U-937 cells in a dose- and time-dependent fashion. Eluates from autopolymerized resins yielded higher percentages of apoptosis and necrosis than the heat-polymerized ones. The results support our hypothesis that eluates of poly(methyl methacrylate)-based denture base acrylic resins activate death-signaling pathways, and that the extent of this process reflects their biocompatibility degree.
M R Cimpan; L I Cressey; N Skaug; A Halstensen; S A Lie; B T Gjertsen; R Matre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of oral sciences     Volume:  108     ISSN:  0909-8836     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-09     Completed Date:  2000-03-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  59-69     Citation Subset:  D; IM    
Institute of Odontology-Department of Dental Biomaterials, Faculty of Dentistry, University of Bergen, Norway.
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MeSH Terms
Annexin A5 / diagnostic use
Apoptosis / drug effects*
Biocompatible Materials / toxicity
Cell Membrane / drug effects
DNA Fragmentation
Denture Bases / adverse effects*
Dose-Response Relationship, Drug
Flow Cytometry
Linear Models
Materials Testing
Microscopy, Electron
Polymethyl Methacrylate / chemistry,  toxicity*
Propidium / diagnostic use
U937 Cells
Reg. No./Substance:
0/Annexin A5; 0/Biocompatible Materials; 36015-30-2/Propidium; 9011-14-7/Polymethyl Methacrylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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